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-Structure paper
タイトル | The structure of the yeast mitochondrial ribosome. |
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ジャーナル・号・ページ | Science, Vol. 355, Issue 6324, Page 528-531, Year 2017 |
掲載日 | 2017年2月3日 |
![]() | Nirupa Desai / Alan Brown / Alexey Amunts / V Ramakrishnan / ![]() ![]() |
PubMed 要旨 | Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the ...Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the structure of the 75-component yeast mitoribosome to an overall resolution of 3.3 angstroms. The mitoribosomal small subunit has been built de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in the evolutionarily related bacterial ribosome. Yeast-specific rRNA and protein elements, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architecture compared to the mammalian mitoribosome. At an expanded messenger RNA channel exit, there is a binding platform for translational activators that regulate translation in yeast but not mammalian mitochondria. The structure provides insights into the evolution and species-specific specialization of mitochondrial translation. |
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手法 | EM (単粒子) |
解像度 | 3.2 - 4.97 Å |
構造データ | EMDB-3551, PDB-5mrc: EMDB-3552, PDB-5mre: EMDB-3553, PDB-5mrf: ![]() EMDB-3554: ![]() EMDB-3555: ![]() EMDB-3556: |
化合物 | ![]() ChemComp-MG: ![]() ChemComp-NA: ![]() ChemComp-ZN: ![]() ChemComp-GDP: |
由来 |
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![]() | RIBOSOME / yeast / mitochondrial / saccharomyces cerevisiae |