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-Structure paper
タイトル | Cryo-EM structure of a human spliceosome activated for step 2 of splicing. |
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ジャーナル・号・ページ | Nature, Vol. 542, Issue 7641, Page 318-323, Year 2017 |
掲載日 | 2017年2月16日 |
著者 | Karl Bertram / Dmitry E Agafonov / Wen-Ti Liu / Olexandr Dybkov / Cindy L Will / Klaus Hartmuth / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann / |
PubMed 要旨 | Spliceosome rearrangements facilitated by RNA helicase PRP16 before catalytic step two of splicing are poorly understood. Here we report a 3D cryo-electron microscopy structure of the human ...Spliceosome rearrangements facilitated by RNA helicase PRP16 before catalytic step two of splicing are poorly understood. Here we report a 3D cryo-electron microscopy structure of the human spliceosomal C complex stalled directly after PRP16 action (C*). The architecture of the catalytic U2-U6 ribonucleoprotein (RNP) core of the human C* spliceosome is very similar to that of the yeast pre-Prp16 C complex. However, in C* the branched intron region is separated from the catalytic centre by approximately 20 Å, and its position close to the U6 small nuclear RNA ACAGA box is stabilized by interactions with the PRP8 RNase H-like and PRP17 WD40 domains. RNA helicase PRP22 is located about 100 Å from the catalytic centre, suggesting that it destabilizes the spliced mRNA after step two from a distance. Comparison of the structure of the yeast C and human C* complexes reveals numerous RNP rearrangements that are likely to be facilitated by PRP16, including a large-scale movement of the U2 small nuclear RNP. |
リンク | Nature / PubMed:28076346 |
手法 | EM (単粒子) |
解像度 | 5.9 - 8.4 Å |
構造データ | EMDB-3545, PDB-5mqf: EMDB-3547: |
由来 |
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キーワード | SPLICING / Spliceosome / pre-mRNA splicing / macromolecular complex |