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-Structure paper
タイトル | Structural insights into the functional cycle of the ATPase module of the 26S proteasome. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 114, Issue 6, Page 1305-1310, Year 2017 |
掲載日 | 2017年2月7日 |
著者 | Marc Wehmer / Till Rudack / Florian Beck / Antje Aufderheide / Günter Pfeifer / Jürgen M Plitzko / Friedrich Förster / Klaus Schulten / Wolfgang Baumeister / Eri Sakata / |
PubMed 要旨 | In eukaryotic cells, the ubiquitin-proteasome system (UPS) is responsible for the regulated degradation of intracellular proteins. The 26S holocomplex comprises the core particle (CP), where ...In eukaryotic cells, the ubiquitin-proteasome system (UPS) is responsible for the regulated degradation of intracellular proteins. The 26S holocomplex comprises the core particle (CP), where proteolysis takes place, and one or two regulatory particles (RPs). The base of the RP is formed by a heterohexameric AAA ATPase module, which unfolds and translocates substrates into the CP. Applying single-particle cryo-electron microscopy (cryo-EM) and image classification to samples in the presence of different nucleotides and nucleotide analogs, we were able to observe four distinct conformational states (s1 to s4). The resolution of the four conformers allowed for the construction of atomic models of the AAA ATPase module as it progresses through the functional cycle. In a hitherto unobserved state (s4), the gate controlling access to the CP is open. The structures described in this study allow us to put forward a model for the 26S functional cycle driven by ATP hydrolysis. |
リンク | Proc Natl Acad Sci U S A / PubMed:28115689 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.1 - 7.8 Å |
構造データ | |
化合物 | ChemComp-ATP: ChemComp-MG: ChemComp-ADP: ChemComp-ANP: |
由来 |
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キーワード | HYDROLASE / Macromolecular complex / 26S proteasome / Protease |