EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis for the activation of human spliceosome.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 6348, Year 2024
掲載日	2024年7月27日
著者	Xiechao Zhan / Yichen Lu / Yigong Shi /
PubMed 要旨	The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic ...The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic spliceosome (B complex) into the activated spliceosome (B complex) and the catalytically activated spliceosome (B complex), involves major flux of protein components and structural rearrangements. Relying on a splicing inhibitor, we have captured six intermediate states between the B and B complexes: pre-B, B-I, B-II, B-III, B-IV, and post-B. Their cryo-EM structures, together with an improved structure of the catalytic step I spliceosome (C complex), reveal how the catalytic center matures around the internal stem loop of U6 snRNA, how the branch site approaches 5'-splice site, how the RNA helicase PRP2 rearranges to bind pre-mRNA, and how U2 snRNP undergoes remarkable movement to facilitate activation. We identify a previously unrecognized key role of PRP2 in spliceosome activation. Our study recapitulates a molecular choreography of the human spliceosome during its catalytic activation.
リンク	Nat Commun / PubMed:39068178 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 4.2 Å
構造データ	35105 8i0p EMDB-35105, PDB-8i0p: The cryo-EM structure of human pre-Bact complex 手法: EM (単粒子) / 解像度: 3.4 Å 35107 8i0r EMDB-35107, PDB-8i0r: The cryo-EM structure of human Bact-I complex 手法: EM (単粒子) / 解像度: 3.0 Å 35108 8i0s EMDB-35108, PDB-8i0s: The cryo-EM structure of human Bact-II complex 手法: EM (単粒子) / 解像度: 4.2 Å 35109 8i0t EMDB-35109, PDB-8i0t: The cryo-EM structure of human Bact-III complex 手法: EM (単粒子) / 解像度: 3.0 Å 35110 8i0u EMDB-35110, PDB-8i0u: The cryo-EM structure of human Bact-IV complex 手法: EM (単粒子) / 解像度: 3.3 Å 35111 8i0v EMDB-35111, PDB-8i0v: The cryo-EM structure of human post-Bact complex 手法: EM (単粒子) / 解像度: 3.0 Å 35113 8i0w EMDB-35113, PDB-8i0w: The cryo-EM structure of human C complex 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-IHP*: INOSITOL HEXAKISPHOSPHATE / フィチン酸
由来	homo sapiens (ヒト) unidentified adenovirus (ウイルス)
キーワード	SPLICING / spliceosome / pre-Bact complex / RNA splicing / PRP2 / activation / Bact-I complex / Bact-II complex / branching / Bact complex / PRP16