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-Structure paper
タイトル | Structural insights into the decoding capability of isoleucine tRNAs with lysidine and agmatidine. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 31, Issue 5, Page 817-825, Year 2024 |
掲載日 | 2024年3月27日 |
著者 | Naho Akiyama / Kensuke Ishiguro / Takeshi Yokoyama / Kenjyo Miyauchi / Asuteka Nagao / Mikako Shirouzu / Tsutomu Suzuki / |
PubMed 要旨 | The anticodon modifications of transfer RNAs (tRNAs) finetune the codon recognition on the ribosome for accurate translation. Bacteria and archaea utilize the modified cytidines, lysidine (L) and ...The anticodon modifications of transfer RNAs (tRNAs) finetune the codon recognition on the ribosome for accurate translation. Bacteria and archaea utilize the modified cytidines, lysidine (L) and agmatidine (agmC), respectively, in the anticodon of tRNA to decipher AUA codon. L and agmC contain long side chains with polar termini, but their functions remain elusive. Here we report the cryogenic electron microscopy structures of tRNAs recognizing the AUA codon on the ribosome. Both modifications interact with the third adenine of the codon via a unique C-A geometry. The side chains extend toward 3' direction of the mRNA, and the polar termini form hydrogen bonds with 2'-OH of the residue 3'-adjacent to the AUA codon. Biochemical analyses demonstrated that AUA decoding is facilitated by the additional interaction between the polar termini of the modified cytidines and 2'-OH of the fourth mRNA residue. We also visualized cyclic N-threonylcarbamoyladenosine (ctA), another tRNA modification, and revealed a molecular basis how ctA contributes to efficient decoding. |
リンク | Nat Struct Mol Biol / PubMed:38538915 |
手法 | EM (単粒子) |
解像度 | 2.32 - 2.69 Å |
構造データ | EMDB-35001, PDB-8hsp: EMDB-35020, PDB-8htz: EMDB-35022, PDB-8hu1: |
化合物 | ChemComp-MG: ChemComp-K: |
由来 |
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キーワード | RIBOSOME / tRNA modification / decoding |