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-Structure paper
タイトル | Translational termination without a stop codon. |
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ジャーナル・号・ページ | Science, Vol. 354, Issue 6318, Page 1437-1440, Year 2016 |
掲載日 | 2016年12月16日 |
著者 | Nathan R James / Alan Brown / Yuliya Gordiyenko / V Ramakrishnan / |
PubMed 要旨 | Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA) ...Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA). We used electron cryomicroscopy to determine structures of ArfA bound to the ribosome with 3'-truncated mRNA, at resolutions ranging from 3.0 to 3.4 angstroms. ArfA binds within the ribosomal mRNA channel and substitutes for the absent stop codon in the A site by specifically recruiting release factor 2 (RF2), initially in a compact preaccommodated state. A similar conformation of RF2 may occur on stop codons, suggesting a general mechanism for release-factor-mediated translational termination in which a conformational switch leads to peptide release only when the appropriate signal is present in the A site. |
リンク | Science / PubMed:27934701 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.97 - 3.35 Å |
構造データ | EMDB-3489, PDB-5mdv: EMDB-3490, PDB-5mdw: |
化合物 | ChemComp-MG: ChemComp-FME: ChemComp-ZN: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / ribosome ArfA RF2 trans-translation 70S 50S 30S rescue termination cryo-EM / ArfA RF2 trans-translation / 70S / 50S / 30S / rescue termination / cryo-EM / ArfA / RF2 / trans-translation |