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-Structure paper
タイトル | Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover. |
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ジャーナル・号・ページ | Commun Biol, Vol. 6, Issue 1, Page 755, Year 2023 |
掲載日 | 2023年7月28日 |
著者 | Raymond N Burton-Smith / Chihong Song / Hiroshi Ueno / Takeshi Murata / Ryota Iino / Kazuyoshi Murata / |
PubMed 要旨 | The vacuolar-type ATPase from Enterococcus hirae (EhV-ATPase) is a thus-far unique adaptation of V-ATPases, as it performs Na transport and demonstrates an off-axis rotor assembly. Recent single ...The vacuolar-type ATPase from Enterococcus hirae (EhV-ATPase) is a thus-far unique adaptation of V-ATPases, as it performs Na transport and demonstrates an off-axis rotor assembly. Recent single molecule studies of the isolated V domain have indicated that there are subpauses within the three major states of the pseudo three-fold symmetric rotary enzyme. However, there was no structural evidence for these. Herein we activate the EhV-ATPase complex with ATP and identified multiple structures consisting of a total of six states of this complex by using cryo-electron microscopy. The orientations of the rotor complex during turnover, especially in the intermediates, are not as perfectly uniform as expected. The densities in the nucleotide binding pockets in the V domain indicate the different catalytic conditions for the six conformations. The off-axis rotor and its' interactions with the stator a-subunit during rotation suggests that this non-uniform rotor rotation is performed through the entire complex. |
リンク | Commun Biol / PubMed:37507515 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.8 - 7.7 Å |
構造データ | EMDB-34139: E. hirae V-ATPase state 1 (whole complex) EMDB-34140: E. hirae V-ATPase state 1' (whole complex) EMDB-34141: E. hirae V-ATPase state 2 (whole complex) EMDB-34142: E. hirae V-ATPase state 2' (whole complex) EMDB-34143: E. hirae V-ATPase state 3 (whole complex) EMDB-34144: E. hirae V-ATPase state 3' (whole complex) EMDB-34145: E. hirae V-ATPase state 1 (V1 domain) EMDB-34146: E. hirae V-ATPase state 1' (V1 domain) EMDB-34147: E. hirae V-ATPase state 2 (V1 domain) EMDB-34148: E. hirae V-ATPase state 2' (V1 domain) EMDB-34149: E. hirae V-ATPase state 3 (V1 domain) EMDB-34150: E. hirae V-ATPase state 3' (V1 domain) |
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