EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Nasal delivery of single-domain antibody improves symptoms of SARS-CoV-2 infection in an animal model.
ジャーナル・号・ページ	PLoS Pathog, Vol. 17, Issue 10, Page e1009542, Year 2021
掲載日	2021年10月14日
著者	Kei Haga / Reiko Takai-Todaka / Yuta Matsumura / Chihong Song / Tomomi Takano / Takuto Tojo / Atsushi Nagami / Yuki Ishida / Hidekazu Masaki / Masayuki Tsuchiya / Toshiki Ebisudani / Shinya Sugimoto / Toshiro Sato / Hiroyuki Yasuda / Koichi Fukunaga / Akihito Sawada / Naoto Nemoto / Kazuyoshi Murata / Takuya Morimoto / Kazuhiko Katayama /
PubMed 要旨	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the disease COVID-19 can lead to serious symptoms, such as severe pneumonia, in the elderly and those with underlying ...The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the disease COVID-19 can lead to serious symptoms, such as severe pneumonia, in the elderly and those with underlying medical conditions. While vaccines are now available, they do not work for everyone and therapeutic drugs are still needed, particularly for treating life-threatening conditions. Here, we showed nasal delivery of a new, unmodified camelid single-domain antibody (VHH), termed K-874A, effectively inhibited SARS-CoV-2 titers in infected lungs of Syrian hamsters without causing weight loss and cytokine induction. In vitro studies demonstrated that K-874A neutralized SARS-CoV-2 in both VeroE6/TMPRSS2 and human lung-derived alveolar organoid cells. Unlike other drug candidates, K-874A blocks viral membrane fusion rather than viral attachment. Cryo-electron microscopy revealed K-874A bound between the receptor binding domain and N-terminal domain of the virus S protein. Further, infected cells treated with K-874A produced fewer virus progeny that were less infective. We propose that direct administration of K-874A to the lung could be a new treatment for preventing the reinfection of amplified virus in COVID-19 patients.
リンク	PLoS Pathog / PubMed:34648602 / PubMed Central
手法	EM (単粒子)
解像度	3.9 - 6.9 Å
構造データ	EMDB-31572: Minor cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs , focused refinement of K-874A, RBD and NTD 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-31573: Minor cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs 手法: EM (単粒子) / 解像度: 4.4 Å 31574 7fg2 EMDB-31574, PDB-7fg2: Minor cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHH, composite map 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-31575: Major cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs, focussed refinement of K-874A, RBD and NTD 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-31576: Major cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs 手法: EM (単粒子) / 解像度: 3.9 Å 31577 7fg3 EMDB-31577, PDB-7fg3: Major cryo-EM structure of S protein trimer of SARS-CoV2 with K-874, composite map 手法: EM (単粒子) / 解像度: 3.9 Å 31578 7fg7 EMDB-31578, PDB-7fg7: Cryo-EM structure of S protein trimer of SARS-CoV2 手法: EM (単粒子) / 解像度: 6.9 Å
由来	Severe acute respiratory syndrome-related coronavirus (ウイルス) severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト)
キーワード	PROTEIN BINDING / Antibody