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-Structure paper
タイトル | Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation. |
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ジャーナル・号・ページ | Cell, Vol. 164, Issue 1-2, Page 91-9102, Year 2016 |
掲載日 | 2016年1月14日 |
著者 | Basil Johannes Greber / Stefan Gerhardy / Alexander Leitner / Marc Leibundgut / Michèle Salem / Daniel Boehringer / Nicolas Leulliot / Ruedi Aebersold / Vikram Govind Panse / Nenad Ban / |
PubMed 要旨 | Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, ...Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the proofreading of functional centers of the 60S subunit and the release of several ribosome biogenesis factors. We report the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution. In addition to the network of interactions formed by Alb1, the structure reveals a mechanism for ensuring the integrity of the ribosomal polypeptide exit tunnel. Arx1 probes the entire set of inner-ring proteins surrounding the tunnel exit, and the C terminus of Rei1 is deeply inserted into the ribosomal tunnel, where it forms specific contacts along almost its entire length. We provide genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation. |
リンク | Cell / PubMed:26709046 |
手法 | EM (単粒子) |
解像度 | 3.4 - 3.91 Å |
構造データ | EMDB-3151: Cryo-EM Structure of the 60S-Arx1-Alb1-Rei1 Complex EMDB-3152: Cryo-EM structure of the 60S-Arx1-Alb1-Rei1 complex (Rei1 N-terminal His6-tag) EMDB-3153: |
化合物 | ChemComp-MG: ChemComp-ZN: |
由来 |
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キーワード | RIBOSOME / EUKARYOTIC RIBOSOME BIOGENESIS / RIBOSOME MATURATION / RIBOSOME BIOGENESIS FACTOR / 60S RIBOSOMAL SUBUNIT / REI1 / ARX1 / ALB1 / CRYO-EM / RIBOSOMAL POLYPEPTIDE EXIT TUNNEL PROOFREADING |