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-Structure paper
タイトル | The structural basis of function and regulation of neuronal cotransporters NKCC1 and KCC2. |
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ジャーナル・号・ページ | Commun Biol, Vol. 4, Issue 1, Page 226, Year 2021 |
掲載日 | 2021年2月17日 |
著者 | Sensen Zhang / Jun Zhou / Yuebin Zhang / Tianya Liu / Perrine Friedel / Wei Zhuo / Suma Somasekharan / Kasturi Roy / Laixing Zhang / Yang Liu / Xianbin Meng / Haiteng Deng / Wenwen Zeng / Guohui Li / Biff Forbush / Maojun Yang / |
PubMed 要旨 | NKCC and KCC transporters mediate coupled transport of Na+K+Cl and K+Cl across the plasma membrane, thus regulating cell Cl concentration and cell volume and playing critical roles in transepithelial ...NKCC and KCC transporters mediate coupled transport of Na+K+Cl and K+Cl across the plasma membrane, thus regulating cell Cl concentration and cell volume and playing critical roles in transepithelial salt and water transport and in neuronal excitability. The function of these transporters has been intensively studied, but a mechanistic understanding has awaited structural studies of the transporters. Here, we present the cryo-electron microscopy (cryo-EM) structures of the two neuronal cation-chloride cotransporters human NKCC1 (SLC12A2) and mouse KCC2 (SLC12A5), along with computational analysis and functional characterization. These structures highlight essential residues in ion transport and allow us to propose mechanisms by which phosphorylation regulates transport activity. |
リンク | Commun Biol / PubMed:33597714 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.52 - 3.8 Å |
構造データ | EMDB-30542, PDB-7d10: EMDB-30543, PDB-7d14: |
化合物 | ChemComp-PLM: |
由来 |
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キーワード | MEMBRANE PROTEIN / transporter |