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-Structure paper
タイトル | Structural basis of assembly and torque transmission of the bacterial flagellar motor. |
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ジャーナル・号・ページ | Cell, Vol. 184, Issue 10, Page 2665-2679.e19, Year 2021 |
掲載日 | 2021年5月13日 |
著者 | Jiaxing Tan / Xing Zhang / Xiaofei Wang / Caihuang Xu / Shenghai Chang / Hangjun Wu / Ting Wang / Huihui Liang / Haichun Gao / Yan Zhou / Yongqun Zhu / |
PubMed 要旨 | The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key ...The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key determinant of pathogenicity. The detailed structure of the flagellar motor remains unknown. Here we present an atomic-resolution cryoelectron microscopy (cryo-EM) structure of the bacterial flagellar motor complexed with the hook, consisting of 175 subunits with a molecular mass of approximately 6.3 MDa. The structure reveals that 10 peptides protruding from the MS ring with the FlgB and FliE subunits mediate torque transmission from the MS ring to the rod and overcome the symmetry mismatch between the rotational and helical structures in the motor. The LP ring contacts the distal rod and applies electrostatic forces to support its rotation and torque transmission to the hook. This work provides detailed molecular insights into the structure, assembly, and torque transmission mechanisms of the flagellar motor. |
リンク | Cell / PubMed:33882274 |
手法 | EM (単粒子) |
解像度 | 2.8 - 4.5 Å |
構造データ | EMDB-30335, PDB-7cbl: EMDB-30336, PDB-7cbm: EMDB-30348, PDB-7cg0: EMDB-30350, PDB-7cg4: EMDB-30351, PDB-7cg7: EMDB-30354, PDB-7cgb: EMDB-30359, PDB-7cgo: EMDB-31006, PDB-7e80: EMDB-31007, PDB-7e81: EMDB-31008, PDB-7e82: |
化合物 | ChemComp-OCA: |
由来 |
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キーワード | MOTOR PROTEIN / Flagella / LP ring / FlgH / FlgI / Distal rod / FlgG / FlgF / Proximal rod / FlgC / FlgB / FliF / Export apparatus / FliE / MS ring / Hook / FlgE / Hook-basal body / Rod / Motor-hook |