+検索条件
-Structure paper
タイトル | Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC. |
---|---|
ジャーナル・号・ページ | Sci Adv, Vol. 6, Issue 44, Year 2020 |
掲載日 | 2020年10月30日 |
![]() | Fengjiang Liu / Jingxi Liang / Bing Zhang / Yan Gao / Xiuna Yang / Tianyu Hu / Haitao Yang / Wenqing Xu / Luke W Guddat / Zihe Rao / ![]() ![]() |
PubMed 要旨 | In bacteria, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) importers are essential for the uptake of nutrients including the nonreducing disaccharide trehalose, a metabolite that is crucial ...In bacteria, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) importers are essential for the uptake of nutrients including the nonreducing disaccharide trehalose, a metabolite that is crucial for the survival and virulence of several human pathogens including SugABC is an ABC transporter that translocates trehalose from the periplasmic lipoprotein LpqY into the cytoplasm of mycobacteria. Here, we report four high-resolution cryo-electron microscopy structures of the mycobacterial LpqY-SugABC complex to reveal how it binds and passes trehalose through the membrane to the cytoplasm. A unique feature observed in this system is the initial mode of capture of the trehalose at the LpqY interface. Uptake is achieved by a pivotal rotation of LpqY relative to SugABC, moving from an open and accessible conformation to a clamped conformation upon trehalose binding. These findings enrich our understanding as to how ABC transporters facilitate substrate transport across the membrane in Gram-positive bacteria. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 3.3 - 3.78 Å |
構造データ | EMDB-30327, PDB-7cad: EMDB-30328, PDB-7cae: EMDB-30329, PDB-7caf: EMDB-30330, PDB-7cag: |
化合物 | ![]() ChemComp-MG: ![]() ChemComp-ATP: |
由来 |
|
![]() | TRANSPORT PROTEIN / ABC transporter / Lipoprotein / Mycobacteria / Trehalose / Trehalose-specific importer |