EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A general mechanism for transcription bubble nucleation in bacteria.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 120, Issue 14, Page e2220874120, Year 2023
掲載日	2023年4月4日
著者	Andreas U Mueller / James Chen / Mengyu Wu / Courtney Chiu / B Tracy Nixon / Elizabeth A Campbell / Seth A Darst /
PubMed 要旨	Bacterial transcription initiation requires σ factors for nucleation of the transcription bubble. The canonical housekeeping σ factor, σ, nucleates DNA melting via recognition of conserved bases ...Bacterial transcription initiation requires σ factors for nucleation of the transcription bubble. The canonical housekeeping σ factor, σ, nucleates DNA melting via recognition of conserved bases of the promoter -10 motif, which are unstacked and captured in pockets of σ. By contrast, the mechanism of transcription bubble nucleation and formation during the unrelated σ-mediated transcription initiation is poorly understood. Herein, we combine structural and biochemical approaches to establish that σ, like σ, captures a flipped, unstacked base in a pocket formed between its N-terminal region I (RI) and extra-long helix features. Strikingly, RI inserts into the nascent bubble to stabilize the nucleated bubble prior to engagement of the obligate ATPase activator. Our data suggest a general paradigm of transcription initiation that requires σ factors to nucleate an early melted intermediate prior to productive RNA synthesis.
リンク	Proc Natl Acad Sci U S A / PubMed:36972428 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.1 Å
構造データ	EMDB-28783: EsN-dhsU36mm2 full map (map 1) 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-28784: EsN-dhsU36mm2 local map (map 2) 手法: EM (単粒子) / 解像度: 3.0 Å 28785 8f1j EMDB-28785: EsN-dhsU36mm2 composite map PDB-8f1j: SigN RNA polymerase early-melted intermediate bound to mismatch DNA fragment dhsU36mm2 (-12A) 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-28786: EsN-dhsU36duplex full map (map 1) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-28790: EsN-dhsU36duplex local map (map 2) 手法: EM (単粒子) / 解像度: 3.1 Å 28791 8f1k EMDB-28791: EsN-dhsU36duplex composite map PDB-8f1k: SigN RNA polymerase early-melted intermediate bound to full duplex DNA fragment dhsU36 (-12T) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-28792: EsN-dhsU36mm1 full map (map 1) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-28794: EsN-dhsU36mm1 local map (map 2) 手法: EM (単粒子) / 解像度: 3.0 Å 28797 8f1i EMDB-28797: EsN-dhsU36mm1 composite map PDB-8f1i: SigN RNA polymerase early-melted intermediate bound to mismatch fragment dhsU36mm1 (-12T) 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER / 水
由来	escherichia coli (大腸菌) aquifex aeolicus (バクテリア)
キーワード	TRANSCRIPTION/DNA / promoter-bound / initiation / DNA melting / transcription bubble nucleation / TRANSCRIPTION (転写 (生物学)) / TRANSCRIPTION-DNA complex