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-Structure paper
タイトル | Brominated lipid probes expose structural asymmetries in constricted membranes. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 30, Issue 2, Page 167-175, Year 2023 |
掲載日 | 2023年1月9日 |
![]() | Frank R Moss / James Lincoff / Maxwell Tucker / Arshad Mohammed / Michael Grabe / Adam Frost / ![]() |
PubMed 要旨 | Lipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and ...Lipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and a model ESCRT-III membrane-remodeling system composed of human CHMP1B and IST1, we observed leaflet-level and protein-localized structural lipid patterns within highly constricted and thinned membrane nanotubes. These nanotubes differed markedly from protein-free, flat bilayers in leaflet thickness, lipid diffusion rates and lipid compositional and conformational asymmetries. Simulations and cryo-EM imaging of brominated stearoyl-docosahexanenoyl-phosphocholine showed how a pair of phenylalanine residues scored the outer leaflet with a helical hydrophobic defect where polyunsaturated docosahexaenoyl tails accumulated at the bilayer surface. Combining cryo-EM of halogenated lipids with molecular dynamics thus enables new characterizations of the composition and structure of membranes on molecular length scales. |
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手法 | EM (らせん対称) |
解像度 | 2.8 - 9.5 Å |
構造データ | ![]() EMDB-27991: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated SDPC probe ![]() EMDB-28694: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 with brominated SDPC probe ![]() EMDB-28695: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated POPS probe ![]() EMDB-28696: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated POPS probe ![]() EMDB-28697: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated PIP2 probe ![]() EMDB-28698: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated PIP2 probe ![]() EMDB-28699: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated cholesterol probe ![]() EMDB-28700: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9L/F13L)+IST1 filament with no brominated lipids ![]() EMDB-28701: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with no brominated lipids ![]() EMDB-28702: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with iodinated cholesterol probe ![]() EMDB-28703: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with iodinated cholesterol probe ![]() EMDB-28704: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 25% brominated SDPC probe ![]() EMDB-28705: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with 25% brominated SDPC probe ![]() EMDB-28706: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 50% brominated SDPC probe ![]() EMDB-28707: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with 50% brominated SDPC probe ![]() EMDB-28708: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 0% cholesterol ![]() EMDB-28709: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 10% cholesterol ![]() EMDB-28710: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 20% cholesterol ![]() EMDB-28711: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 30% cholesterol ![]() EMDB-28712: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 40% cholesterol ![]() EMDB-28713: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9A/F13A)+IST1 filament with brominated SDPC probe ![]() EMDB-28714: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9A/F133A)+IST1 filament with brominated SDPC probe ![]() EMDB-28715: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9E/F13E)+IST1 filament with brominated SDPC probe ![]() EMDB-28716: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9E/F13E)+IST1 filament with brominated SDPC probe ![]() EMDB-28717: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9L/F13L)+IST1 filament with brominated SDPC probe ![]() EMDB-28718: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9L/F13L)+IST1 filament with brominated SDPC probe ![]() EMDB-28719: CryoEM reconstruction of membrane-bound, right-handed, 34-subunit-per-turn CHMP1B+IST1 filament with POPC substituted for SDPC ![]() EMDB-28722: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated cholesterol probe |
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