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-Structure paper
タイトル | Structure, folding and flexibility of co-transcriptional RNA origami. |
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ジャーナル・号・ページ | Nat Nanotechnol, Vol. 18, Issue 7, Page 808-817, Year 2023 |
掲載日 | 2023年2月27日 |
著者 | Ewan K S McRae / Helena Østergaard Rasmussen / Jianfang Liu / Andreas Bøggild / Michael T A Nguyen / Nestor Sampedro Vallina / Thomas Boesen / Jan Skov Pedersen / Gang Ren / Cody Geary / Ebbe Sloth Andersen / |
PubMed 要旨 | RNA origami is a method for designing RNA nanostructures that can self-assemble through co-transcriptional folding with applications in nanomedicine and synthetic biology. However, to advance the ...RNA origami is a method for designing RNA nanostructures that can self-assemble through co-transcriptional folding with applications in nanomedicine and synthetic biology. However, to advance the method further, an improved understanding of RNA structural properties and folding principles is required. Here we use cryogenic electron microscopy to study RNA origami sheets and bundles at sub-nanometre resolution revealing structural parameters of kissing-loop and crossover motifs, which are used to improve designs. In RNA bundle designs, we discover a kinetic folding trap that forms during folding and is only released after 10 h. Exploration of the conformational landscape of several RNA designs reveal the flexibility of helices and structural motifs. Finally, sheets and bundles are combined to construct a multidomain satellite shape, which is characterized by individual-particle cryo-electron tomography to reveal the domain flexibility. Together, the study provides a structural basis for future improvements to the design cycle of genetically encoded RNA nanodevices. |
リンク | Nat Nanotechnol / PubMed:36849548 / PubMed Central |
手法 | EM (単粒子) / EM (トモグラフィー) |
解像度 | 4.08 - 27.0 Å |
構造データ | EMDB-13592: Cryo-EM SPA reconstruction of an extended RNA origami 5 helix tile (5HT-B-3X) EMDB-13625: Mature conformer 2 of a 6-helix bundle of RNA with a clasp EMDB-13626: Young conformer 2 of a 6-helix bundle of RNA with a clasp EMDB-13627: 6-Helix bundle of RNA EMDB-13628, PDB-7ptk: EMDB-13630, PDB-7ptl: EMDB-13633, PDB-7ptq: EMDB-13636, PDB-7pts: EMDB-13926, PDB-7qdu: EMDB-28669: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 01) EMDB-28670: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 02) EMDB-28671: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 03) EMDB-28672: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 04) EMDB-28673: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 05) EMDB-28674: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 06) EMDB-28675: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 07) EMDB-28676: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 08) EMDB-28677: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 09) EMDB-28678: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 10) EMDB-28679: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 11) EMDB-28680: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 12) EMDB-28681: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 13) EMDB-28682: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 14) EMDB-28683: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 15) EMDB-28684: Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 16) |
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キーワード | RNA / origami / nanostructure |