EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational trajectory of allosteric gating of the human cone photoreceptor cyclic nucleotide-gated channel.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4284, Year 2023
掲載日	2023年7月18日
著者	Zhengshan Hu / Xiangdong Zheng / Jian Yang /
PubMed 要旨	Cyclic nucleotide-gated (CNG) channels transduce chemical signals into electrical signals in sensory receptors and neurons. They are activated by cGMP or cAMP, which bind to the cyclic nucleotide- ...Cyclic nucleotide-gated (CNG) channels transduce chemical signals into electrical signals in sensory receptors and neurons. They are activated by cGMP or cAMP, which bind to the cyclic nucleotide-binding domain (CNBD) to open a gate located 50-60 Å away in the central cavity. Structures of closed and open vertebrate CNG channels have been solved, but the conformational landscape of this allosteric gating remains to be elucidated and enriched. Here, we report structures of the cGMP-activated human cone photoreceptor CNGA3/CNGB3 channel in closed, intermediate, pre-open and open states in detergent or lipid nanodisc, all with fully bound cGMP. The pre-open and open states are obtained only in the lipid nanodisc, suggesting a critical role of lipids in tuning the energetic landscape of CNGA3/CNGB3 activation. The different states exhibit subunit-unique, incremental and distinct conformational rearrangements that originate in the CNBD, propagate through the gating ring to the transmembrane domain, and gradually open the S6 cavity gate. Our work illustrates a spatial conformational-change wave of allosteric gating of a vertebrate CNG channel by its natural ligand and provides an expanded framework for studying CNG properties and channelopathy.
リンク	Nat Commun / PubMed:37463923 / PubMed Central
手法	EM (単粒子)
解像度	3.11 - 3.62 Å
構造データ	28595 8etp EMDB-28595, PDB-8etp: Cryo-EM structure of cGMP bound closed state of human CNGA3/CNGB3 channel in GDN 手法: EM (単粒子) / 解像度: 3.52 Å 28603 8eu3 EMDB-28603, PDB-8eu3: Cryo-EM structure of cGMP bound human CNGA3/CNGB3 channel in GDN, transition state 1 手法: EM (単粒子) / 解像度: 3.62 Å 28611 8euc EMDB-28611, PDB-8euc: Cryo-EM structure of cGMP bound human CNGA3/CNGB3 channel in GDN, transition state 2 手法: EM (単粒子) / 解像度: 3.61 Å 28622 8ev8 EMDB-28622, PDB-8ev8: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, closed state 手法: EM (単粒子) / 解像度: 3.11 Å 28623 8ev9 EMDB-28623, PDB-8ev9: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1 手法: EM (単粒子) / 解像度: 3.33 Å 28624 8eva EMDB-28624, PDB-8eva: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 2 手法: EM (単粒子) / 解像度: 3.33 Å 28625 8evb EMDB-28625, PDB-8evb: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, pre-open state 手法: EM (単粒子) / 解像度: 3.6 Å 28626 8evc EMDB-28626, PDB-8evc: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, open state 手法: EM (単粒子) / 解像度: 3.33 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-PCG: CYCLIC GUANOSINE MONOPHOSPHATE / cGMP
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / Heterotetramer / ligand-bound / ligand-gated / ion channel / CNGA3 / CNGB3