EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of LRP2 reveal a molecular machine for endocytosis.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 4, Page 821-836.e13, Year 2023
掲載日	2023年2月16日
著者	Andrew Beenken / Gabriele Cerutti / Julia Brasch / Yicheng Guo / Zizhang Sheng / Hediye Erdjument-Bromage / Zainab Aziz / Shelief Y Robbins-Juarez / Estefania Y Chavez / Goran Ahlsen / Phinikoula S Katsamba / Thomas A Neubert / Anthony W P Fitzpatrick / Jonathan Barasch / Lawrence Shapiro /
PubMed 要旨	The low-density lipoprotein (LDL) receptor-related protein 2 (LRP2 or megalin) is representative of the phylogenetically conserved subfamily of giant LDL receptor-related proteins, which function in ...The low-density lipoprotein (LDL) receptor-related protein 2 (LRP2 or megalin) is representative of the phylogenetically conserved subfamily of giant LDL receptor-related proteins, which function in endocytosis and are implicated in diseases of the kidney and brain. Here, we report high-resolution cryoelectron microscopy structures of LRP2 isolated from mouse kidney, at extracellular and endosomal pH. The structures reveal LRP2 to be a molecular machine that adopts a conformation for ligand binding at the cell surface and for ligand shedding in the endosome. LRP2 forms a homodimer, the conformational transformation of which is governed by pH-sensitive sites at both homodimer and intra-protomer interfaces. A subset of LRP2 deleterious missense variants in humans appears to impair homodimer assembly. These observations lay the foundation for further understanding the function and mechanism of LDL receptors and implicate homodimerization as a conserved feature of the LRP receptor subfamily.
リンク	Cell / PubMed:36750096 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.3 Å
構造データ	28233 8em4 EMDB-28233, PDB-8em4: Cryo-EM structure of LRP2 at pH 7.5 手法: EM (単粒子) / 解像度: 2.83 Å 28241 8em7 EMDB-28241, PDB-8em7: Cryo-EM structure of LRP2 at pH 5.2 手法: EM (単粒子) / 解像度: 2.97 Å EMDB-28242: Local refinement map of LRP2 P1-P2 domains at pH 7.5 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-28243: Local refinement of P3-P6 domains of LRP2 at pH 7.5 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-28250: Local refinement of the P7 domain of LRP2 at pH 7.5 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-28251: Local refinement of the R4 domain of LRP2 at pH 7.5 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-28252: Local refinement of P8 domain of LRP2 at pH 7.5 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-28253: Local refinement of P1-P2 domains of LRP2 at pH 5.2 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-28258: Local refinement of P3-P6 domains of LRP2 at pH 5.2 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-28260: Local refinement of P7 domain of LRP2 at pH 5.2 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-28261: Local refinement of R3 domain of LRP2 at pH 5.2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-28265: Local refinement of P8 domain of LRP2 at pH 5.2 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-NGA: 2-acetamido-2-deoxy-beta-D-galactopyranose / 2-(アセチルアミノ)-2-デオキシ-β-D-ガラクトピラノ-ス ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	mus musculus (ハツカネズミ) house mouse (ハツカネズミ)
キーワード	MEMBRANE PROTEIN / LRP2 / Megalin / GP330 / Endocytosis