EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transient Prenyltransferase-Cyclase Association in Fusicoccadiene Synthase, an Assembly-Line Terpene Synthase.
ジャーナル・号・ページ	Biochemistry, Vol. 61, Issue 21, Page 2417-2430, Year 2022
掲載日	2022年11月1日
著者	Jacque L Faylo / Trevor van Eeuwen / Kushol Gupta / Kenji Murakami / David W Christianson /
PubMed 要旨	Fusicoccadiene synthase from the fungus (PaFS) is an assembly-line terpene synthase that catalyzes the first two steps in the biosynthesis of Fusiccocin A, a diterpene glycoside. The C-terminal ...Fusicoccadiene synthase from the fungus (PaFS) is an assembly-line terpene synthase that catalyzes the first two steps in the biosynthesis of Fusiccocin A, a diterpene glycoside. The C-terminal prenyltransferase domain of PaFS catalyzes the condensation of one molecule of C dimethylallyl diphosphate and three molecules of C isopentenyl diphosphate to form C geranylgeranyl diphosphate, which then transits to the cyclase domain for cyclization to form fusicoccadiene. Previous structural studies of PaFS using electron microscopy (EM) revealed a central octameric prenyltransferase core with eight cyclase domains tethered in random distal positions through flexible 70-residue linkers. However, proximal prenyltransferase-cyclase configurations could be captured by covalent cross-linking and observed by cryo-EM and mass spectrometry. Here, we use cryo-EM to show that proximally configured prenyltransferase-cyclase complexes are observable even in the absence of covalent cross-linking; moreover, such complexes can involve multiple cyclase domains. A conserved basic patch on the prenyltransferase domain comprises the primary touchpoint with the cyclase domain. These results support a model for transient prenyltransferase-cyclase association in which the cyclase domains of PaFS are in facile equilibrium between proximal associated and random distal positions relative to the central prenyltransferase octamer. The results of biophysical measurements using small-angle X-ray scattering, analytical ultracentrifugation, dynamic light scattering, and size-exclusion chromatography in-line with multi-angle light scattering are consistent with this model. This model accordingly provides a framework for understanding substrate transit between the prenyltransferase and cyclase domains as well as the cooperativity observed for geranylgeranyl diphosphate cyclization.
リンク	Biochemistry / PubMed:36227241 / PubMed Central
手法	EM (単粒子)
解像度	3.73 - 8.4 Å
構造データ	27989 8eax EMDB-27989, PDB-8eax: Octameric prenyltransferase domain of fusicoccadiene Synthase with C2 symmetry sans transiently associating cyclase domains 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-28046: Symmetry-expanded consensus map of PaFS prenyltransferase core with single interacting cyclase domain 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-28076: Symmetry-expanded map of PaFS prenyltransferase core with single interacting cyclase domain class A 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-28079: Symmetry-expanded map of PaFS prenyltransferase core with single interacting cyclase domain, class B 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-28131: PaFS prenyltransferase core with single interacting cyclase domain - North class A 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-28132: PaFS prenyltransferase core with single interacting cyclase domain - North class D 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-28191: PaFS prenyltransferase core with single interacting cyclase domain - North class C 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-28193: PaFS prenyltransferase core with single interacting cyclase domain - North class B 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-28194: PaFS prenyltransferase core with single interacting cyclase domain - East class D 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-28201: PaFS prenyltransferase core with single interacting cyclase domain - East class B 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-28202: PaFS prenyltransferase core with single interacting cyclase domain - East class A 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-28203: PaFS prenyltransferase core with single interacting cyclase domain - East class C 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-28213: PaFS prenyltransferase core with single interacting cyclase domain - South class A 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-28215: PaFS prenyltransferase core with single interacting cyclase domain - South class B 手法: EM (単粒子) / 解像度: 7.9 Å EMDB-28216: PaFS prenyltransferase core with single interacting cyclase domain - South class C 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-28219: PaFS prenyltransferase core with single interacting cyclase domain - West class A 手法: EM (単粒子) / 解像度: 7.9 Å EMDB-28220: PaFS prenyltransferase core with single interacting cyclase domain - West class B 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-28221: PaFS prenyltransferase core with single interacting cyclase domain - West class C 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-28222: PaFS prenyltransferase core with single interacting cyclase domain - West class D 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-28235: PaFS prenyltransferase core with two interacting cyclase domains - East and South 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-28236: PaFS prenyltransferase core with two interacting cyclase domains - South and East 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-28237: PaFS prenyltransferase core with two interacting cyclase domains - East and West 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-28239: PaFS prenyltransferase core with two interacting cyclase domains - West and East 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-28240: PaFS prenyltransferase core with two interacting cyclase domains - North and East 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-28245: PaFS prenyltransferase core with two interacting cyclase domains - East and North 手法: EM (単粒子) / 解像度: 6.9 Å
由来	diaporthe amygdali (菌類) Escherichia coli (大腸菌) Diaporthe amygdali
キーワード	TRANSFERASE / Terpene synthase / Prenyltransferase