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-Structure paper
タイトル | Structures of human primosome elongation complexes. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 30, Issue 5, Page 579-583, Year 2023 |
掲載日 | 2023年4月17日 |
著者 | Qixiang He / Andrey G Baranovskiy / Lucia M Morstadt / Alisa E Lisova / Nigar D Babayeva / Benjamin L Lusk / Ci Ji Lim / Tahir H Tahirov / |
PubMed 要旨 | The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. ...The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers. |
リンク | Nat Struct Mol Biol / PubMed:37069376 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.35 - 3.59 Å |
構造データ | EMDB-27256, PDB-8d96: EMDB-27258, PDB-8d9d: |
化合物 | ChemComp-SF4: ChemComp-MG: ChemComp-DTP: ChemComp-ZN: |
由来 |
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キーワード | REPLICATION / DNA replication / human DNA polymerase alpha/primase / human primosome / elongation complex |