EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for non-canonical integrin engagement by Bordetella adenylate cyclase toxin.
ジャーナル・号・ページ	Cell Rep, Vol. 40, Issue 7, Page 111196, Year 2022
掲載日	2022年8月16日
著者	Jory A Goldsmith / Andrea M DiVenere / Jennifer A Maynard / Jason S McLellan /
PubMed 要旨	Integrins are ubiquitous cell-surface heterodimers that are exploited by pathogens and toxins, including leukotoxins that target β integrins on phagocytes. The Bordetella adenylate cyclase toxin ...Integrins are ubiquitous cell-surface heterodimers that are exploited by pathogens and toxins, including leukotoxins that target β integrins on phagocytes. The Bordetella adenylate cyclase toxin (ACT) uses the αβ integrin as a receptor, but the structural basis for integrin binding and neutralization by antibodies is poorly understood. Here, we use cryoelectron microscopy to determine a 2.7 Å resolution structure of an ACT fragment bound to αβ. This structure reveals that ACT interacts with the headpiece and calf-2 of the α subunit in a non-canonical manner specific to bent, inactive αβ. Neutralizing antibody epitopes map to ACT residues involved in α binding, providing the basis for antibody-mediated attachment inhibition. Furthermore, binding to αβ positions the essential ACT acylation sites, which are conserved among toxins exported by type I secretion systems, at the cell membrane. These findings reveal a structural mechanism for integrin-mediated attachment and explain antibody-mediated neutralization of ACT intoxication.
リンク	Cell Rep / PubMed:35977491 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.1 Å
構造データ	26738 7usl EMDB-26738, PDB-7usl: Integrin alphaM/beta2 ectodomain in complex with adenylate cyclase toxin RTX751 and M1F5 Fab 手法: EM (単粒子) / 解像度: 2.7 Å 26739 7usm EMDB-26739, PDB-7usm: Integrin alphaM/beta2 ectodomain 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-27122: Global refinement for integrin alphaM/beta2 ectodomain in complex with adenylate cyclase toxin RTX751 and M1F5 Fab 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-27123: Tailpiece local refinement for integrin alphaM/beta2 ectodomain in complex with adenylate cyclase toxin RTX751 and M1F5 Fab 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-27124: Acylation domain local refinement for integrin alphaM/beta2 ectodomain in complex with adenylate cyclase toxin RTX751 and M1F5 Fab 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-27125: Global refinement for integrin alphaM/beta2 ectodomain 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-27126: Tailpiece local refinement for integrin alphaM/beta2 ectodomain 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	homo sapiens (ヒト) bordetella pertussis (百日咳菌)
キーワード	TOXIN / Receptor / Complex / Integrin / CELL ADHESION / Complement