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-Structure paper
タイトル | Visualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 4802, Year 2022 |
掲載日 | 2022年8月15日 |
著者 | Simon Jenni / Joshua A Horwitz / Louis-Marie Bloyet / Sean P J Whelan / Stephen C Harrison / |
PubMed 要旨 | Vesicular stomatitis virus (VSV) is a negative-strand RNA virus with a non-segmented genome, closely related to rabies virus. Both have characteristic bullet-like shapes. We report the structure of ...Vesicular stomatitis virus (VSV) is a negative-strand RNA virus with a non-segmented genome, closely related to rabies virus. Both have characteristic bullet-like shapes. We report the structure of intact, infectious VSV particles determined by cryogenic electron microscopy. By compensating for polymorphism among viral particles with computational classification, we obtained a reconstruction of the shaft ("trunk") at 3.5 Å resolution, with lower resolution for the rounded tip. The ribonucleoprotein (RNP), genomic RNA complexed with nucleoprotein (N), curls into a dome-like structure with about eight gradually expanding turns before transitioning into the regular helical trunk. Two layers of matrix (M) protein link the RNP with the membrane. Radial inter-layer subunit contacts are fixed within single RNA-N-M1-M2 modules, but flexible lateral and axial interactions allow assembly of polymorphic virions. Together with published structures of recombinant N in various states, our results suggest a mechanism for membrane-coupled self-assembly of VSV and its relatives. |
リンク | Nat Commun / PubMed:35970826 / PubMed Central |
手法 | EM (らせん対称) / EM (単粒子) |
解像度 | 3.5 - 4.1 Å |
構造データ | EMDB-26602, PDB-7umk: EMDB-26603, PDB-7uml: |
由来 |
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キーワード | VIRUS / Vesicular stomatitis virus / bullet-shaped particle / Rhrabdovirus / nucleocapsid / helical reconstruction / cryo electron microscopy / nucleoprotein / matrix protein / RNA / assembly / VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA complex |