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-Structure paper
タイトル | ε, a new subunit of RNA polymerase found in gram-positive bacteria. |
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ジャーナル・号・ページ | J Bacteriol, Vol. 196, Issue 20, Page 3622-3632, Year 2014 |
掲載日 | 2014年8月4日 |
著者 | Andrew N Keller / Xiao Yang / Jana Wiedermannová / Olivier Delumeau / Libor Krásný / Peter J Lewis / |
PubMed 要旨 | RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T Firmicutes phylum of ...RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T Firmicutes phylum of Gram-positive bacteria and have named it ε. Previously ε had been identified as a small protein (ω1) that copurified with RNA polymerase. We have solved the structure of ε by X-ray crystallography and show that it is not an ω subunit. Rather, ε bears remarkable similarity to the Gp2 family of phage proteins involved in the inhibition of host cell transcription following infection. Deletion of ε shows no phenotype and has no effect on the transcriptional profile of the cell. Determination of the location of ε within the assembly of RNA polymerase core by single-particle analysis suggests that it binds toward the downstream side of the DNA binding cleft. Due to the structural similarity of ε with Gp2 and the fact they bind similar regions of RNA polymerase, we hypothesize that ε may serve a role in protection from phage infection. |
リンク | J Bacteriol / PubMed:25092033 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.3 - 24.0 Å |
構造データ | EMDB-2637: PDB-4njc: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | TRANSCRIPTION / DNA Dependant RNA Polymerase |