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-Structure paper
タイトル | Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition. |
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ジャーナル・号・ページ | Mol Cell, Vol. 83, Issue 11, Page 1827-11838.e6, Year 2023 |
掲載日 | 2023年6月1日 |
著者 | Jung-Un Park / Michael T Petassi / Shan-Chi Hsieh / Eshan Mehrotra / Gabriel Schuler / Jagat Budhathoki / Vinh H Truong / Summer B Thyme / Ailong Ke / Elizabeth H Kellogg / Joseph E Peters / |
PubMed 要旨 | CRISPR-associated transposons (CASTs) are natural RNA-directed transposition systems. We demonstrate that transposon protein TniQ plays a central role in promoting R-loop formation by RNA-guided DNA- ...CRISPR-associated transposons (CASTs) are natural RNA-directed transposition systems. We demonstrate that transposon protein TniQ plays a central role in promoting R-loop formation by RNA-guided DNA-targeting modules. TniQ residues, proximal to CRISPR RNA (crRNA), are required for recognizing different crRNA categories, revealing an unappreciated role of TniQ to direct transposition into different classes of crRNA targets. To investigate adaptations allowing CAST elements to utilize attachment sites inaccessible to CRISPR-Cas surveillance complexes, we compared and contrasted PAM sequence requirements in both I-F3b CAST and I-F1 CRISPR-Cas systems. We identify specific amino acids that enable a wider range of PAM sequences to be accommodated in I-F3b CAST elements compared with I-F1 CRISPR-Cas, enabling CAST elements to access attachment sites as sequences drift and evade host surveillance. Together, this evidence points to the central role of TniQ in facilitating the acquisition of CRISPR effector complexes for RNA-guided DNA transposition. |
リンク | Mol Cell / PubMed:37267904 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.52 - 4.03 Å |
構造データ | EMDB-26348, PDB-7u5d: EMDB-26349: I-F3b Cascade-TniQ full R-loop complex |
由来 |
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キーワード | DNA BINDING PROTEIN/DNA/RNA / CRISPR-Cas / Transposon / CAST / Cascade / I-F / I-F3 / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA-RNA complex |