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-Structure paper
タイトル | A public antibody class recognizes an S2 epitope exposed on open conformations of SARS-CoV-2 spike. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 4539, Year 2022 |
掲載日 | 2022年8月4日 |
![]() | Mathieu Claireaux / Tom G Caniels / Marlon de Gast / Julianna Han / Denise Guerra / Gius Kerster / Barbera D C van Schaik / Aldo Jongejan / Angela I Schriek / Marloes Grobben / Philip J M Brouwer / Karlijn van der Straten / Yoann Aldon / Joan Capella-Pujol / Jonne L Snitselaar / Wouter Olijhoek / Aafke Aartse / Mitch Brinkkemper / Ilja Bontjer / Judith A Burger / Meliawati Poniman / Tom P L Bijl / Jonathan L Torres / Jeffrey Copps / Isabel Cuella Martin / Steven W de Taeye / Godelieve J de Bree / Andrew B Ward / Kwinten Sliepen / Antoine H C van Kampen / Perry D Moerland / Rogier W Sanders / Marit J van Gils / ![]() ![]() |
PubMed 要旨 | Delineating the origins and properties of antibodies elicited by SARS-CoV-2 infection and vaccination is critical for understanding their benefits and potential shortcomings. Therefore, we ...Delineating the origins and properties of antibodies elicited by SARS-CoV-2 infection and vaccination is critical for understanding their benefits and potential shortcomings. Therefore, we investigate the SARS-CoV-2 spike (S)-reactive B cell repertoire in unexposed individuals by flow cytometry and single-cell sequencing. We show that ∼82% of SARS-CoV-2 S-reactive B cells harbor a naive phenotype, which represents an unusually high fraction of total human naive B cells (∼0.1%). Approximately 10% of these naive S-reactive B cells share an IGHV1-69/IGKV3-11 B cell receptor pairing, an enrichment of 18-fold compared to the complete naive repertoire. Following SARS-CoV-2 infection, we report an average 37-fold enrichment of IGHV1-69/IGKV3-11 B cell receptor pairing in the S-reactive memory B cells compared to the unselected memory repertoire. This class of B cells targets a previously undefined non-neutralizing epitope on the S2 subunit that becomes exposed on S proteins used in approved vaccines when they transition away from the native pre-fusion state because of instability. These findings can help guide the improvement of SARS-CoV-2 vaccines. |
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手法 | EM (単粒子) |
解像度 | 13.12 - 18.5 Å |
構造データ | ![]() EMDB-26217: Negative stain EM map of COVA1-07 mAb bound to the S2 domain of SARS-CoV-2 S ![]() EMDB-26218: Negative stain EM map of COVA2-14 mAb bound to the S2 domain of SARS-CoV-2 S ![]() EMDB-26219: Negative stain EM map of COVA2-18 mAb bound to the S2 domain of SARS-CoV-2 S ![]() EMDB-26220: Negative stain EM map of the S2 domain of SARS-CoV-2 S |
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