EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The RIG-I receptor adopts two different conformations for distinguishing host from viral RNA ligands.
ジャーナル・号・ページ	Mol Cell, Vol. 82, Issue 21, Page 4131-44144.e6, Year 2022
掲載日	2022年11月3日
著者	Wenshuai Wang / Anna Marie Pyle /
PubMed 要旨	RIG-I is an essential innate immune receptor for detecting and responding to infection by RNA viruses. RIG-I specifically recognizes the unique molecular features of viral RNA molecules and ...RIG-I is an essential innate immune receptor for detecting and responding to infection by RNA viruses. RIG-I specifically recognizes the unique molecular features of viral RNA molecules and selectively distinguishes them from closely related RNAs abundant in host cells. The physical basis for this exquisite selectivity is revealed through a series of high-resolution cryo-EM structures of RIG-I in complex with host and viral RNA ligands. These studies demonstrate that RIG-I actively samples double-stranded RNAs in the cytoplasm and distinguishes them by adopting two different types of protein folds. Upon binding viral RNA, RIG-I adopts a high-affinity conformation that is conducive to signaling, while host RNA induces an autoinhibited conformation that stimulates RNA release. By coupling protein folding with RNA binding selectivity, RIG-I distinguishes RNA molecules that differ by as little as one phosphate group, thereby explaining the molecular basis for selective antiviral sensing and the induction of autoimmunity upon RIG-I dysregulation.
リンク	Mol Cell / PubMed:36272408 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.66 Å
構造データ	26022 7tnx EMDB-26022, PDB-7tnx: Cryo-EM structure of RIG-I in complex with p3dsRNA 手法: EM (単粒子) / 解像度: 3.54 Å 26023 7tny EMDB-26023, PDB-7tny: Cryo-EM structure of RIG-I in complex with p2dsRNA 手法: EM (単粒子) / 解像度: 3.2 Å 26024 7tnz EMDB-26024, PDB-7tnz: Cryo-EM structure of RIG-I in complex with p1dsRNA 手法: EM (単粒子) / 解像度: 3.54 Å 26025 7to0 EMDB-26025, PDB-7to0: Cryo-EM structure of RIG-I in complex with OHdsRNA 手法: EM (単粒子) / 解像度: 3.5 Å 26026 7to1 EMDB-26026, PDB-7to1: Cryo-EM structure of RIG-I bound to the end of p3SLR30 (+ATP) 手法: EM (単粒子) / 解像度: 3.66 Å 26027 7to2 EMDB-26027, PDB-7to2: Cryo-EM structure of RIG-I bound to the internal sites of p3SLR30 (+ATP) 手法: EM (単粒子) / 解像度: 3.2 Å 27743 8dvr EMDB-27743, PDB-8dvr: Cryo-EM structure of RIG-I bound to the end of p3SLR30 (+AMPPNP) 手法: EM (単粒子) / 解像度: 3.3 Å 27744 8dvs EMDB-27744, PDB-8dvs: Cryo-EM structure of RIG-I bound to the end of OHSLR30 (+ATP) 手法: EM (単粒子) / 解像度: 3.0 Å 27745 8dvu EMDB-27745, PDB-8dvu: Cryo-EM structure of RIG-I bound to the internal sites of OHSLR30 (+ATP) 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM
由来	homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM/RNA / ribonucleoprotein complex / RNA sensor / RIG-I like receptor / IMMUNE SYSTEM / IMMUNE SYSTEM-RNA complex / Hydrolase/RNA / Hydrolase-RNA complex