EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Broadly neutralizing antibodies target a haemagglutinin anchor epitope.
ジャーナル・号・ページ	Nature, Vol. 602, Issue 7896, Page 314-320, Year 2022
掲載日	2021年12月23日
著者	Jenna J Guthmiller / Julianna Han / Henry A Utset / Lei Li / Linda Yu-Ling Lan / Carole Henry / Christopher T Stamper / Meagan McMahon / George O'Dell / Monica L Fernández-Quintero / Alec W Freyn / Fatima Amanat / Olivia Stovicek / Lauren Gentles / Sara T Richey / Alba Torrents de la Peña / Victoria Rosado / Haley L Dugan / Nai-Ying Zheng / Micah E Tepora / Dalia J Bitar / Siriruk Changrob / Shirin Strohmeier / Min Huang / Adolfo García-Sastre / Klaus R Liedl / Jesse D Bloom / Raffael Nachbagauer / Peter Palese / Florian Krammer / Lynda Coughlan / Andrew B Ward / Patrick C Wilson /
PubMed 要旨	Broadly neutralizing antibodies that target epitopes of haemagglutinin on the influenza virus have the potential to provide near universal protection against influenza virus infection. However, viral ...Broadly neutralizing antibodies that target epitopes of haemagglutinin on the influenza virus have the potential to provide near universal protection against influenza virus infection. However, viral mutants that escape broadly neutralizing antibodies have been reported. The identification of broadly neutralizing antibody classes that can neutralize viral escape mutants is critical for universal influenza virus vaccine design. Here we report a distinct class of broadly neutralizing antibodies that target a discrete membrane-proximal anchor epitope of the haemagglutinin stalk domain. Anchor epitope-targeting antibodies are broadly neutralizing across H1 viruses and can cross-react with H2 and H5 viruses that are a pandemic threat. Antibodies that target this anchor epitope utilize a highly restricted repertoire, which encodes two public binding motifs that make extensive contacts with conserved residues in the fusion peptide. Moreover, anchor epitope-targeting B cells are common in the human memory B cell repertoire and were recalled in humans by an oil-in-water adjuvanted chimeric haemagglutinin vaccine, which is a potential universal influenza virus vaccine. To maximize protection against seasonal and pandemic influenza viruses, vaccines should aim to boost this previously untapped source of broadly neutralizing antibodies that are widespread in the human memory B cell pool.
リンク	Nature / PubMed:34942633 / PubMed Central
手法	EM (単粒子)
解像度	3.38 - 20.0 Å
構造データ	EMDB-25634: Negative stain map of monoclonal Fab 047-09 4F04 binding the anchor epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25635: Negative stain map of monoclonal Fab 241 IgA 2F04 binding the anchor epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25636: Negative stain map of polyclonal Fab 236.7 binding the anchor and esterase epitopes of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25637: Negative stain map of polyclonal Fab 236.7 binding the RBS epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25638: Negative stain map of polyclonal Fab 236.14 binding an epitope on the top of the head of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25639: Negative stain map of polyclonal Fab 236.14 binding the esterase epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25640: Negative stain map of polycolonal Fab 236.14 binding the RBS epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25641: Negative stain map of polyclonal Fab 236.14 binding the anchor epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25642: Negative stain map of polyclonal Fab 241.7 binding the esterase epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25643: Negative stain map of polyclonal Fab 241.14 binding the anchor epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25644: Negative stain map of polyclonal Fab 241.14 binding the esterase epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25645: Negative stain map of polyclonal Fab 241.14 binding an epitope on the top of the head of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-25646: Negative stain map of polyclonal Fab 241.14 binding the RBS epitope of H1 HA 手法: EM (単粒子) / 解像度: 20.0 Å 25655 7t3d EMDB-25655, PDB-7t3d: CryoEM map of anchor 222-1C06 Fab and lateral patch 2B05 Fab binding H1 HA 手法: EM (単粒子) / 解像度: 3.38 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	homo sapiens (ヒト) influenza a virus (a/california/04/2009(h1n1)) (A型インフルエンザウイルス)
キーワード	Viral Protein/IMMUNE SYSTEM (ウイルス性) / anchor (錨) / antibodies (抗体) / influenza A virus (A型インフルエンザウイルス) / hemagglutinin (ヘマグルチニン) / IMMUNE SYSTEM (免疫系) / Viral Protein-IMMUNE SYSTEM complex (ウイルス性)