EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural mechanism of muscle nicotinic receptor desensitization and block by curare.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 4, Page 386-394, Year 2022
掲載日	2022年3月17日
著者	Md Mahfuzur Rahman / Tamara Basta / Jinfeng Teng / Myeongseon Lee / Brady T Worrell / Michael H B Stowell / Ryan E Hibbs /
PubMed 要旨	Binding of the neurotransmitter acetylcholine to its receptors on muscle fibers depolarizes the membrane and thereby triggers muscle contraction. We sought to understand at the level of three- ...Binding of the neurotransmitter acetylcholine to its receptors on muscle fibers depolarizes the membrane and thereby triggers muscle contraction. We sought to understand at the level of three-dimensional structure how agonists and antagonists alter nicotinic acetylcholine receptor conformation. We used the muscle-type receptor from the Torpedo ray to first define the structure of the receptor in a resting, activatable state. We then determined the receptor structure bound to the agonist carbachol, which stabilizes an asymmetric, closed channel desensitized state. We find conformational changes in a peripheral membrane helix are tied to recovery from desensitization. To probe mechanisms of antagonism, we obtained receptor structures with the active component of curare, a poison arrow toxin and precursor to modern muscle relaxants. d-Tubocurarine stabilizes the receptor in a desensitized-like state in the presence and absence of agonist. These findings define the transitions between resting and desensitized states and reveal divergent means by which antagonists block channel activity of the muscle-type nicotinic receptor.
リンク	Nat Struct Mol Biol / PubMed:35301478 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.18 Å
構造データ	25202 7smm EMDB-25202, PDB-7smm: Cryo-EM structure of Torpedo acetylcholine receptor in apo form 手法: EM (単粒子) / 解像度: 2.5 Å 25205 7smq EMDB-25205, PDB-7smq: Cryo-EM structure of Torpedo acetylcholine receptor in apo form with added cholesterol 手法: EM (単粒子) / 解像度: 2.74 Å 25206 7smr EMDB-25206, PDB-7smr: Cryo-EM structure of Torpedo acetylcholine receptor in complex with carbachol, desensitized state 手法: EM (単粒子) / 解像度: 2.77 Å 25207 7sms EMDB-25207, PDB-7sms: Cryo-EM structure of Torpedo acetylcholine receptor in complex with d-tubocurarine 手法: EM (単粒子) / 解像度: 3.18 Å 25208 7smt EMDB-25208, PDB-7smt: Cryo-EM structure of Torpedo acetylcholine receptor in complex with d-tubocurarine and carbachol 手法: EM (単粒子) / 解像度: 2.56 Å
化合物	ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-DD9: nonane / ノナン ChemComp-HOH: WATER ChemComp-CCE: 2-[(AMINOCARBONYL)OXY]-N,N,N-TRIMETHYLETHANAMINIUM / カルバミルコリン ChemComp-TC9: D-TUBOCURARINE / alkaloidYM
由来	tetronarce californica (エイ) Pacific electric ray (エイ) Pacific electric ray, Torpedo californica
キーワード	TRANSPORT PROTEIN / acetylcholine receptor / nicotinic receptor / Torpedo / Cys-loop receptor / ion channel / muscle-type nicotinic receptor