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-Structure paper
タイトル | Cryo-EM structure of the sodium-driven chloride/bicarbonate exchanger NDCBE. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 5690, Year 2021 |
掲載日 | 2021年9月28日 |
著者 | Weiguang Wang / Kirill Tsirulnikov / Hristina R Zhekova / Gülru Kayık / Hanif Muhammad Khan / Rustam Azimov / Natalia Abuladze / Liyo Kao / Debbie Newman / Sergei Yu Noskov / Z Hong Zhou / Alexander Pushkin / Ira Kurtz / |
PubMed 要旨 | SLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) ...SLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) transporters revealed an identical overall fold despite their different transport modes (chloride/bicarbonate exchange versus sodium-carbonate cotransport). However, the exact mechanism determining the different transport modes in the SLC4 family remains unknown. In this work, we report the cryo-EM 3.4 Å structure of the OF conformation of NDCBE (SLC4A8), which shares transport properties with both AE1 and NBCe1 by mediating the electroneutral exchange of sodium-carbonate with chloride. This structure features a fully resolved extracellular loop 3 and well-defined densities corresponding to sodium and carbonate ions in the tentative substrate binding pocket. Further, we combine computational modeling with functional studies to unravel the molecular determinants involved in NDCBE and SLC4 transport. |
リンク | Nat Commun / PubMed:34584093 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.4 Å |
構造データ | EMDB-24683, PDB-7rtm: |
化合物 | ChemComp-NAG: ChemComp-NA: ChemComp-CO3: |
由来 |
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キーワード | MEMBRANE PROTEIN / NDCBE / sodium-driven chloride/bicarbonate exchanger / cryo-EM |