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-Structure paper
| タイトル | Structural basis for isoform-specific inhibition of human CTPS1. |
|---|---|
| ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 118, Issue 40, Year 2021 |
| 掲載日 | 2021年10月5日 |
 著者 | Eric M Lynch / Michael A DiMattia / Steven Albanese / Gydo C P van Zundert / Jesse M Hansen / Joel D Quispe / Madison A Kennedy / Andreas Verras / Kenneth Borrelli / Angela V Toms / Neelu Kaila / Kevin D Kreutter / Joshua J McElwee / Justin M Kollman /  |
| PubMed 要旨 | Cytidine triphosphate synthase 1 (CTPS1) is necessary for an effective immune response, as revealed by severe immunodeficiency in CTPS1-deficient individuals [E. Martin ], [] [510], [288-292] ([2014]) ...Cytidine triphosphate synthase 1 (CTPS1) is necessary for an effective immune response, as revealed by severe immunodeficiency in CTPS1-deficient individuals [E. Martin ], [] [510], [288-292] ([2014]). CTPS1 expression is up-regulated in activated lymphocytes to expand CTP pools [E. Martin ], [] [510], [288-292] ([2014]), satisfying increased demand for nucleic acid and lipid synthesis [L. D. Fairbanks, M. Bofill, K. Ruckemann, H. A. Simmonds], [ ] [270], [29682-29689] ([1995]). Demand for CTP in other tissues is met by the CTPS2 isoform and nucleoside salvage pathways [E. Martin ], [] [510], [288-292] ([2014]). Selective inhibition of the proliferative CTPS1 isoform is therefore desirable in the treatment of immune disorders and lymphocyte cancers, but little is known about differences in regulation of the isoforms or mechanisms of known inhibitors. We show that CTP regulates both isoforms by binding in two sites that clash with substrates. CTPS1 is less sensitive to CTP feedback inhibition, consistent with its role in increasing CTP levels in proliferation. We also characterize recently reported small-molecule inhibitors, both CTPS1 selective and nonselective. Cryo-electron microscopy (cryo-EM) structures reveal these inhibitors mimic CTP binding in one inhibitory site, where a single amino acid substitution explains selectivity for CTPS1. The inhibitors bind to CTPS assembled into large-scale filaments, which for CTPS1 normally represents a hyperactive form of the enzyme [E. M. Lynch ], [] [24], [507-514] ([2017]). This highlights the utility of cryo-EM in drug discovery, particularly for cases in which targets form large multimeric assemblies not amenable to structure determination by other techniques. Both inhibitors also inhibit the proliferation of human primary T cells. The mechanisms of selective inhibition of CTPS1 lay the foundation for the design of immunosuppressive therapies. |
 リンク | Proc Natl Acad Sci U S A / PubMed:34583994 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.4 - 6.2 Å |
| 構造データ | EMDB-23831, PDB-7mgz: EMDB-23832, PDB-7mh0: EMDB-23833, PDB-7mh1: EMDB-23848, PDB-7mif: EMDB-23850, PDB-7mig: EMDB-23851, PDB-7mih: EMDB-23852, PDB-7mii: EMDB-23859, PDB-7mip: EMDB-23865, PDB-7miu: EMDB-23866, PDB-7miv: |
| 化合物 |  ChemComp-UTP:  ChemComp-GLN:  ChemComp-MG:  ChemComp-ANP:  ChemComp-CTP:  ChemComp-ZG4:  ChemComp-ZFY: |
| 由来 |
|
 キーワード |  LIGASE (リガーゼ) / glutaminase (グルタミナーゼ) / amidoligase / nucleotide metabolism (ヌクレオチド) |
