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-Structure paper
タイトル | Structural and functional dissection of reovirus capsid folding and assembly by the prefoldin-TRiC/CCT chaperone network. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 118, Issue 11, Year 2021 |
掲載日 | 2021年3月16日 |
著者 | Jonathan J Knowlton / Daniel Gestaut / Boxue Ma / Gwen Taylor / Alpay Burak Seven / Alexander Leitner / Gregory J Wilson / Sreejesh Shanker / Nathan A Yates / B V Venkataram Prasad / Ruedi Aebersold / Wah Chiu / Judith Frydman / Terence S Dermody / |
PubMed 要旨 | Intracellular protein homeostasis is maintained by a network of chaperones that function to fold proteins into their native conformation. The eukaryotic TRiC chaperonin (TCP1-ring complex, also ...Intracellular protein homeostasis is maintained by a network of chaperones that function to fold proteins into their native conformation. The eukaryotic TRiC chaperonin (TCP1-ring complex, also called CCT for cytosolic chaperonin containing TCP1) facilitates folding of a subset of proteins with folding constraints such as complex topologies. To better understand the mechanism of TRiC folding, we investigated the biogenesis of an obligate TRiC substrate, the reovirus σ3 capsid protein. We discovered that the σ3 protein interacts with a network of chaperones, including TRiC and prefoldin. Using a combination of cryoelectron microscopy, cross-linking mass spectrometry, and biochemical approaches, we establish functions for TRiC and prefoldin in folding σ3 and promoting its assembly into higher-order oligomers. These studies illuminate the molecular dynamics of σ3 folding and establish a biological function for TRiC in virus assembly. In addition, our findings provide structural and functional insight into the mechanism by which TRiC and prefoldin participate in the assembly of protein complexes. |
リンク | Proc Natl Acad Sci U S A / PubMed:33836586 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.5 - 6.2 Å |
構造データ | EMDB-23522: TRiC-substrate complex in ATP/AlFx closed state EMDB-23526, PDB-7lup: |
化合物 | ChemComp-ZN: ChemComp-HOH: |
由来 |
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キーワード | CHAPERONE / tric / cct / reovirus capsid / sigma 3 |