EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for potassium transport in prokaryotes by KdpFABC.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 29, Year 2021
掲載日	2021年7月20日
著者	Marie E Sweet / Casper Larsen / Xihui Zhang / Michael Schlame / Bjørn P Pedersen / David L Stokes /
PubMed 要旨	KdpFABC is an oligomeric K transport complex in prokaryotes that maintains ionic homeostasis under stress conditions. The complex comprises a channel-like subunit (KdpA) from the superfamily of K ...KdpFABC is an oligomeric K transport complex in prokaryotes that maintains ionic homeostasis under stress conditions. The complex comprises a channel-like subunit (KdpA) from the superfamily of K transporters and a pump-like subunit (KdpB) from the superfamily of P-type ATPases. Recent structural work has defined the architecture and generated contradictory hypotheses for the transport mechanism. Here, we use substrate analogs to stabilize four key intermediates in the reaction cycle and determine the corresponding structures by cryogenic electron microscopy. We find that KdpB undergoes conformational changes consistent with other representatives from the P-type superfamily, whereas KdpA, KdpC, and KdpF remain static. We observe a series of spherical densities that we assign as K or water and which define a pathway for K transport. This pathway runs through an intramembrane tunnel in KdpA and delivers ions to sites in the membrane domain of KdpB. Our structures suggest a mechanism where ATP hydrolysis is coupled to K transfer between alternative sites in KdpB, ultimately reaching a low-affinity site where a water-filled pathway allows release of K to the cytoplasm.
リンク	Proc Natl Acad Sci U S A / PubMed:34272288 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.76 Å
構造データ	12184 7bgy EMDB-12184, PDB-7bgy: Cryo-EM Structure of KdpFABC in E2Pi state with MgF4 手法: EM (単粒子) / 解像度: 2.9 Å 12185 7bh1 EMDB-12185, PDB-7bh1: Cryo-EM Structure of KdpFABC in E1 state with K 手法: EM (単粒子) / 解像度: 3.38 Å 12186 7bh2 EMDB-12186, PDB-7bh2: Cryo-EM Structure of KdpFABC in E2Pi state with BeF3 and K+ 手法: EM (単粒子) / 解像度: 3.0 Å 23268 7lc3 EMDB-23268, PDB-7lc3: CryoEM Structure of KdpFABC in E1-ATP state 手法: EM (単粒子) / 解像度: 3.23 Å 23269 7lc6 EMDB-23269, PDB-7lc6: Cryo-EM Structure of KdpFABC in E2-P state with BeF3 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-23342: Cryo-EM Structure of KdpFABC in E1 state 手法: EM (単粒子) / 解像度: 3.57 Å EMDB-23343: Cryo-EM Structure of KdpFABC in E2Pi state with AlF4 手法: EM (単粒子) / 解像度: 3.32 Å EMDB-23344: CryoEM Structure of KdpFABC in E2Pi state with AlF4 手法: EM (単粒子) / 解像度: 3.76 Å EMDB-23346: CryoEM Structure of KdpFABC in E2Pi state with VO4 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-23347: CryoEM Structure of KdpFABC in E1 state 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-23348: Cryo-EM Structure of KdpFABC in E2Pi state with VO4 and K+ 手法: EM (単粒子) / 解像度: 3.38 Å EMDB-23349: CryoEM Structure of KdpFABC in E1 state 手法: EM (単粒子) / 解像度: 3.32 Å EMDB-23353: Cryo-EM Structure of KdpFABC in E1ATP state with AMP-PCP 手法: EM (単粒子) / 解像度: 3.27 Å EMDB-23354: Cryo-EM Structure of KdpFABC in E2Pi state with BeF3 手法: EM (単粒子) / 解像度: 3.21 Å
化合物	ChemComp-PEV: (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / POPE, リン脂質YM ChemComp-9Y0: (2R)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (E)-octadec-9-enoate ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-MF4: TETRAFLUOROMAGNESATE(2-) ChemComp-HOH: WATER ChemComp-K: Unknown entry / カリウムカチオン ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / β,γ-メチレンATP / AMP-PCP, エネルギー貯蔵分子類似体YM
由来	escherichia coli k-12 (大腸菌) escherichia coli (strain k12) (大腸菌)
キーワード	MEMBRANE PROTEIN / P-type ATPase / ATP-dependent potassium pump