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-Structure paper
タイトル | Structural visualization of key steps in human transcription initiation. |
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ジャーナル・号・ページ | Nature, Vol. 495, Issue 7442, Page 481-486, Year 2013 |
掲載日 | 2013年3月28日 |
著者 | Yuan He / Jie Fang / Dylan J Taatjes / Eva Nogales / |
PubMed 要旨 | Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the ...Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the transcription start site. The molecular mechanism and function of this assembly have remained elusive due to lack of structural information. Here we have used an in vitro reconstituted system to study the stepwise assembly of human TBP, TFIIA, TFIIB, Pol II, TFIIF, TFIIE and TFIIH onto promoter DNA using cryo-electron microscopy. Our structural analyses provide pseudo-atomic models at various stages of transcription initiation that illuminate critical molecular interactions, including how TFIIF engages Pol II and promoter DNA to stabilize both the closed pre-initiation complex and the open-promoter complex, and to regulate start--initiation complexes, combined with the localization of the TFIIH helicases XPD and XPB, support a DNA translocation model of XPB and explain its essential role in promoter opening. |
リンク | Nature / PubMed:23446344 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 11.0 - 20.0 Å |
構造データ | EMDB-2304: EMDB-2305: EMDB-2306: EMDB-2307: EMDB-2308: EMDB-2309: |
由来 |
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