EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Immunofocusing and enhancing autologous Tier-2 HIV-1 neutralization by displaying Env trimers on two-component protein nanoparticles.
ジャーナル・号・ページ	NPJ Vaccines, Vol. 6, Issue 1, Page 24, Year 2021
掲載日	2021年2月9日
著者	Philip J M Brouwer / Aleksandar Antanasijevic / Marlon de Gast / Joel D Allen / Tom P L Bijl / Anila Yasmeen / Rashmi Ravichandran / Judith A Burger / Gabriel Ozorowski / Jonathan L Torres / Celia LaBranche / David C Montefiori / Rajesh P Ringe / Marit J van Gils / John P Moore / Per Johan Klasse / Max Crispin / Neil P King / Andrew B Ward / Rogier W Sanders /
PubMed 要旨	The HIV-1 envelope glycoprotein trimer is poorly immunogenic because it is covered by a dense glycan shield. As a result, recombinant Env glycoproteins generally elicit inadequate antibody levels ...The HIV-1 envelope glycoprotein trimer is poorly immunogenic because it is covered by a dense glycan shield. As a result, recombinant Env glycoproteins generally elicit inadequate antibody levels that neutralize clinically relevant, neutralization-resistant (Tier-2) HIV-1 strains. Multivalent antigen presentation on nanoparticles is an established strategy to increase vaccine-driven immune responses. However, due to nanoparticle instability in vivo, the display of non-native Env structures, and the inaccessibility of many neutralizing antibody (NAb) epitopes, the effects of nanoparticle display are generally modest for Env trimers. Here, we generate two-component self-assembling protein nanoparticles presenting twenty SOSIP trimers of the clade C Tier-2 genotype 16055. We show in a rabbit immunization study that these nanoparticles induce 60-fold higher autologous Tier-2 NAb titers than the corresponding SOSIP trimers. Epitope mapping studies reveal that the presentation of 16055 SOSIP trimers on these nanoparticle focuses antibody responses to an immunodominant apical epitope. Thus, these nanoparticles are a promising platform to improve the immunogenicity of Env trimers with apex-proximate NAb epitopes.
リンク	NPJ Vaccines / PubMed:33563983 / PubMed Central
手法	EM (単粒子)
解像度	12.8 - 17.2 Å
構造データ	EMDB-22714: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2463 手法: EM (単粒子) / 解像度: 15.3 Å EMDB-22715: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2464 手法: EM (単粒子) / 解像度: 15.9 Å EMDB-22716: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2465 手法: EM (単粒子) / 解像度: 15.2 Å EMDB-22717: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2466 手法: EM (単粒子) / 解像度: 15.2 Å EMDB-22718: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2467 手法: EM (単粒子) / 解像度: 17.2 Å EMDB-22719: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2468 手法: EM (単粒子) / 解像度: 15.2 Å EMDB-22720: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2469 手法: EM (単粒子) / 解像度: 12.8 Å EMDB-22721: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2470 手法: EM (単粒子) / 解像度: 13.2 Å EMDB-22722: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2471 手法: EM (単粒子) / 解像度: 15.2 Å EMDB-22723: nsEM map of the 16055 SOSIP HIV Env trimer in complex with polyclonal Fab from rabbit r2472 手法: EM (単粒子) / 解像度: 12.8 Å
由来	synthetic construct (人工物)