EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the host cell recognition and penetration machinery of a Staphylococcus aureus bacteriophage.
ジャーナル・号・ページ	PLoS Pathog, Vol. 16, Issue 2, Page e1008314, Year 2020
掲載日	2020年2月18日
著者	James L Kizziah / Keith A Manning / Altaira D Dearborn / Terje Dokland /
PubMed 要旨	Staphylococcus aureus is a common cause of infections in humans. The emergence of virulent, antibiotic-resistant strains of S. aureus is a significant public health concern. Most virulence and ...Staphylococcus aureus is a common cause of infections in humans. The emergence of virulent, antibiotic-resistant strains of S. aureus is a significant public health concern. Most virulence and resistance factors in S. aureus are encoded by mobile genetic elements, and transduction by bacteriophages represents the main mechanism for horizontal gene transfer. The baseplate is a specialized structure at the tip of bacteriophage tails that plays key roles in host recognition, cell wall penetration, and DNA ejection. We have used high-resolution cryo-electron microscopy to determine the structure of the S. aureus bacteriophage 80α baseplate at 3.75 Å resolution, allowing atomic models to be built for most of the major tail and baseplate proteins, including two tail fibers, the receptor binding protein, and part of the tape measure protein. Our structure provides a structural basis for understanding host recognition, cell wall penetration and DNA ejection in viruses infecting Gram-positive bacteria. Comparison to other phages demonstrates the modular design of baseplate proteins, and the adaptations to the host that take place during the evolution of staphylococci and other pathogens.
リンク	PLoS Pathog / PubMed:32069326 / PubMed Central
手法	EM (単粒子)
解像度	3.45 - 4.14 Å
構造データ	20872 6v8i EMDB-20872: C3 symmetrical reconstruction of the Staphylococcus aureus phage 80alpha baseplate PDB-6v8i: Composite atomic model of the Staphylococcus aureus phage 80alpha baseplate 手法: EM (単粒子) / 解像度: 3.96 Å 20873 6v8i EMDB-20873: C6 symmetrical reconstruction of the Staphylococcus aureus phage 80alpha baseplate PDB-6v8i: Composite atomic model of the Staphylococcus aureus phage 80alpha baseplate 手法: EM (単粒子) / 解像度: 3.45 Å 20874 6v8i EMDB-20874: Lower fiber-focused reconstruction of the Staphylococcus aureus phage 80alpha baseplate PDB-6v8i: Composite atomic model of the Staphylococcus aureus phage 80alpha baseplate 手法: EM (単粒子) / 解像度: 3.69 Å 20875 6v8i EMDB-20875: Upper fiber-focused reconstruction of the Staphylococcus aureus phage 80alpha baseplate PDB-6v8i: Composite atomic model of the Staphylococcus aureus phage 80alpha baseplate 手法: EM (単粒子) / 解像度: 4.14 Å 20876 6v8i EMDB-20876: Receptor binding protein-focused reconstruction of the Staphylococcus aureus phage 80alpha baseplate PDB-6v8i: Composite atomic model of the Staphylococcus aureus phage 80alpha baseplate 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-FE: Unknown entry
由来	staphylococcus virus 80alpha (ウイルス)
キーワード	VIRAL PROTEIN / phage tail / tail tip / tape measure protein