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-Structure paper
タイトル | Molecular model of the human 26S proteasome. |
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ジャーナル・号・ページ | Mol Cell, Vol. 46, Issue 1, Page 54-66, Year 2012 |
掲載日 | 2012年4月13日 |
![]() | Paula C A da Fonseca / Jun He / Edward P Morris / ![]() |
PubMed 要旨 | The 26S proteasome plays a fundamental role in eukaryotic homeostasis by undertaking the highly controlled degradation of a wide range of proteins, including key cellular regulators such as those ...The 26S proteasome plays a fundamental role in eukaryotic homeostasis by undertaking the highly controlled degradation of a wide range of proteins, including key cellular regulators such as those controlling cell-cycle progression and apoptosis. Here we report the structure of the human 26S proteasome determined by cryo-electron microscopy and single-particle analysis, with secondary structure elements identified both in the 20S proteolytic core region and in the 19S regulatory particle. We have used this information together with crystal structures, homology models, and other biochemical information to construct a molecular model of the complete 26S proteasome. This model allows for a detailed description of the 20S core within the 26S proteasome and redefines the overall assignment of subunits within the 19S regulatory particle. The information presented here provides a strong basis for a mechanistic understanding of the 26S proteasome. |
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手法 | EM (単粒子) |
解像度 | 7.0 Å |
構造データ | ![]() EMDB-2047: |
由来 |
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