EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.
ジャーナル・号・ページ	Structure, Vol. 27, Issue 10, Page 1594-11600.e2, Year 2019
掲載日	2019年10月1日
著者	Michael W Martynowycz / Wei Zhao / Johan Hattne / Grant J Jensen / Tamir Gonen /
PubMed 要旨	Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction ...Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED.
リンク	Structure / PubMed:31422911 / PubMed Central
手法	EM (電子線結晶学)
解像度	1.79 - 2.59 Å
構造データ	20356 6pkj EMDB-20356, PDB-6pkj: MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#2) 手法: EM (電子線結晶学) / 解像度: 2.17 Å 20357 6pkk EMDB-20357, PDB-6pkk: MicroED structure of proteinase K from an uncoated, single lamella at 2.18A resolution (#5) 手法: EM (電子線結晶学) / 解像度: 2.176 Å 20358 6pkl EMDB-20358, PDB-6pkl: MicroED structure of proteinase K from an uncoated, single lamella at 2.59A resolution (#7) 手法: EM (電子線結晶学) / 解像度: 2.59 Å 20359 6pkm EMDB-20359, PDB-6pkm: MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8) 手法: EM (電子線結晶学) / 解像度: 2.17 Å 20360 6pkn EMDB-20360, PDB-6pkn: MicroED structure of proteinase K from an unpolished, platinum-coated, single lamella at 2.08A resolution (#9) 手法: EM (電子線結晶学) 20361 6pko EMDB-20361: MicroED structure of proteinase K from a platinum-coated, unpolished, single lamella at 2.07A resolution (#12) PDB-6pko: MicroED structure of proteinase K from a platinum coated, unpolished, single lamella at 2.07A resolution (#12) 手法: EM (電子線結晶学) / 解像度: 2.07 Å 20362 6pkp EMDB-20362, PDB-6pkp: MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.91A resolution (#10) 手法: EM (電子線結晶学) / 解像度: 1.91 Å 20363 6pkq EMDB-20363, PDB-6pkq: MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.85A resolution (#11) 手法: EM (電子線結晶学) / 解像度: 1.85 Å 20364 6pkr EMDB-20364, PDB-6pkr: MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.79A resolution (#13) 手法: EM (電子線結晶学) / 解像度: 1.79 Å 20365 6pks EMDB-20365, PDB-6pks: MicroED structure of proteinase K from low-dose merged lamellae that were not pre-coated with platinum 2.16A resolution (LD) 手法: EM (電子線結晶学) / 解像度: 2.16 Å 20366 6pkt EMDB-20366, PDB-6pkt: MicroED structure of proteinase K from merging low-dose, platinum pre-coated lamellae at 1.85A resolution (LDPT) 手法: EM (電子線結晶学) / 解像度: 1.85 Å
化合物	ChemComp-HOH: WATER
由来	parengyodontium album (菌類) Engyodontium album, Tritirachium album
キーワード	HYDROLASE