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-Structure paper
タイトル | Bubblegrams reveal the inner body of bacteriophage φKZ. |
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ジャーナル・号・ページ | Science, Vol. 335, Issue 6065, Page 182, Year 2012 |
掲載日 | 2012年1月13日 |
著者 | Weimin Wu / Julie A Thomas / Naiqian Cheng / Lindsay W Black / Alasdair C Steven / |
PubMed 要旨 | Dense packing of macromolecules in cellular compartments and higher-order assemblies makes it difficult to pick out even quite large components in electron micrographs, despite nominally high ...Dense packing of macromolecules in cellular compartments and higher-order assemblies makes it difficult to pick out even quite large components in electron micrographs, despite nominally high resolution. Immunogold labeling and histochemical procedures offer ways to map certain components but are limited in their applicability. Here, we present a differential mapping procedure, based on the physical principle of protein's greater sensitivity to radiation damage compared with that of nucleic acid. |
リンク | Science / PubMed:22246767 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 37.0 Å |
構造データ | EMDB-1996: |