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タイトル | Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM. |
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ジャーナル・号・ページ | Nano Lett, Vol. 24, Issue 16, Page 5031-5038, Year 2024 |
掲載日 | 2024年4月11日 |
著者 | Ali Khoshouei / Georg Kempf / Volodymyr Mykhailiuk / Johanna Mariko Griessing / Maximilian Nicolas Honemann / Lukas Kater / Simone Cavadini / Hendrik Dietz / |
PubMed 要旨 | DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular ...DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications. |
リンク | Nano Lett / PubMed:38602296 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.9 - 8.6 Å |
構造データ | EMDB-19767: Structure of a 2873 Scaffold Base DNA Origami V1 EMDB-19769: Structure of a 2873 Scaffold Base DNA Origami V2 EMDB-19770: Structure of a 2873 Scaffold Base DNA Origami V3 EMDB-19775: Structure of a 1033 Scaffold Base DNA Origami Nanostructure V4 with Desalted Purified Staples EMDB-19776: Structure of a 1033 Scaffold Base DNA Origami Nanostructure V4 with HPLC Purified Staples EMDB-19867, PDB-9eoq: EMDB-19874: Refinement Focused on the 1st Body of a 1033 Scaffold-Based DNA Origami Nanostructure V4 with TBA EMDB-19875: Refinement Focused on the 2nd Body of a 1033 Scaffold-Based DNA Origami Nanostructure V4 with TBA EMDB-19876: Refinement Focused on the 3rd Body of a 1033 Scaffold-Based DNA Origami Nanostructure V4 with TBA |
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キーワード | DNA / DNA Origami / Multilayer / Square lattice / 1033 scaffold / TBA |