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-Structure paper
| タイトル | Stepwise assembly and release of Tc toxins from Yersinia entomophaga. |
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| ジャーナル・号・ページ | Nat Microbiol, Vol. 9, Issue 2, Page 405-420, Year 2024 |
| 掲載日 | 2024年2月5日 |
 著者 | Miki Feldmüller / Charles F Ericson / Pavel Afanasyev / Yun-Wei Lien / Gregor L Weiss / Florian Wollweber / Marion Schoof / Mark Hurst / Martin Pilhofer /   |
| PubMed 要旨 | Tc toxins are virulence factors of bacterial pathogens. Although their structure and intoxication mechanism are well understood, it remains elusive where this large macromolecular complex is ...Tc toxins are virulence factors of bacterial pathogens. Although their structure and intoxication mechanism are well understood, it remains elusive where this large macromolecular complex is assembled and how it is released. Here we show by an integrative multiscale imaging approach that Yersinia entomophaga Tc (YenTc) toxin components are expressed only in a subpopulation of cells that are 'primed' with several other potential virulence factors, including filaments of the protease M66/StcE. A phage-like lysis cassette is required for YenTc release; however, before resulting in complete cell lysis, the lysis cassette generates intermediate 'ghost' cells, which may serve as assembly compartments and become packed with assembled YenTc holotoxins. We hypothesize that this stepwise mechanism evolved to minimize the number of cells that need to be killed. The occurrence of similar lysis cassettes in diverse organisms indicates a conserved mechanism for Tc toxin release that may apply to other extracellular macromolecular machines. |
 リンク | Nat Microbiol / PubMed:38316932 / PubMed Central |
| 手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
| 解像度 | 9.89 - 31.1 Å |
| 構造データ |  EMDB-18953: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells  EMDB-18954: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells  EMDB-18955: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells  EMDB-18957: Cryo-electron tomogram of Yersinia entomophaga MH96 cells  EMDB-18958: Cryo-electron tomogram of Yersinia entomophaga MH96 cells  EMDB-18960: Cryo-electron tomogram of Yersinia entomophaga delta LC cells  EMDB-18961: Cryo-electron tomogram of mechanically cryo-milled Yersinia entomophaga delta LC cells  EMDB-18962: Cryo-electron tomogram of a lysate preparation of Yersinia entomophaga delta LC cells  EMDB-18970: Subtomogram average of M66 filaments in Yersinia entomophaga cells  EMDB-18971: Subtomogram average of YenTc-Chi2-sfGFP from Yersinia entomophaga chi2-sfGFP  EMDB-18972: Subtomogram average of YenTc from Yersinia entomophaga MH96  EMDB-19370: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells  EMDB-19371: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells  EMDB-19372: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells  EMDB-19373: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells  EMDB-19374: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells  EMDB-19375: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells  EMDB-19376: Cryo-electron tomogram of Yersinia entomophaga delta LC delta YenTc cells  EMDB-19377: Cryo-electron tomogram of Yersinia entomophaga MH96 cells grown at 37 degrees  EMDB-19378: Cryo-electron tomogram of Yersinia entomophaga delta LC cells grown at 37 degrees  EMDB-19379: Cryo-electron tomogram of Yersinia entomophaga delta LC delta M66 cells  EMDB-19380: Cryo-electron tomogram of Yersinia entomophaga delta LC delta M66 cells  EMDB-19381: Cryo-electron tomogram of a lysate preparation of Yersinia entomophaga delta LC delta M66 cells |
| 由来 |
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Yersinia entomophaga (バクテリア)