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-Structure paper
タイトル | Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. |
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ジャーナル・号・ページ | J Cell Biol, Vol. 223, Issue 6, Year 2024 |
掲載日 | 2024年6月3日 |
著者 | Bettina Zens / Florian Fäßler / Jesse M Hansen / Robert Hauschild / Julia Datler / Victor-Valentin Hodirnau / Vanessa Zheden / Jonna Alanko / Michael Sixt / Florian K M Schur / |
PubMed 要旨 | The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in ...The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-out extraction procedure, and cryo-electron tomography. Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting in a versatile tool closely mimicking ECM environments. This allows us to visualize ECM for the first time in its hydrated, native context. Our data reveal an intricate network of extracellular fibers, their positioning relative to matrix-secreting cells, and previously unresolved structural entities. Our workflow and results add to the structural atlas of the ECM, providing novel insights into its secretion and assembly. |
リンク | J Cell Biol / PubMed:38506714 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-18490: Cryo-electron tomogram of lift-out lamella from cell-derived extracellular matrix (example 1) EMDB-18491: Cryo-electron tomogram of lift-out lamella from cell-derived extracellular matrix (example 2) EMDB-18492: Cryo-electron tomogram of lift-out lamella from cell-derived extracellular matrix (example 3) EMDB-18493: Cryo-electron tomogram of lift-out lamella from cell-derived extracellular matrix (example 4) EMDB-18494: Cryo-electron tomogram of lift-out lamella from cell-derived extracellular matrix (example 5) |
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