EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural characterization of the oligomerization of full-length Hantaan virus polymerase into symmetric dimers and hexamers.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 2256, Year 2024
掲載日	2024年3月13日
著者	Quentin Durieux Trouilleton / Dominique Housset / Paco Tarillon / Benoît Arragain / Hélène Malet /
PubMed 要旨	Hantaan virus is a dangerous human pathogen whose segmented negative-stranded RNA genome is replicated and transcribed by a virally-encoded multi-functional polymerase. Here we describe the complete ...Hantaan virus is a dangerous human pathogen whose segmented negative-stranded RNA genome is replicated and transcribed by a virally-encoded multi-functional polymerase. Here we describe the complete cryo-electron microscopy structure of Hantaan virus polymerase in several oligomeric forms. Apo polymerase protomers can adopt two drastically different conformations, which assemble into two distinct symmetric homodimers, that can themselves gather to form hexamers. Polymerase dimerization induces the stabilization of most polymerase domains, including the C-terminal domain that contributes the most to dimer's interface, along with a lariat region that participates to the polymerase steadying. Binding to viral RNA induces significant conformational changes resulting in symmetric oligomer disruption and polymerase activation, suggesting the possible involvement of apo multimers as protecting systems that would stabilize the otherwise flexible C-terminal domains. Overall, these results provide insights into the multimerization capability of Hantavirus polymerase and may help to define antiviral compounds to counteract these life-threatening viruses.
リンク	Nat Commun / PubMed:38480734 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.6 Å
構造データ	18343 8qe5 EMDB-18343, PDB-8qe5: Apo Hantaan virus polymerase in monomeric state 手法: EM (単粒子) / 解像度: 2.6 Å 18390 8qgt EMDB-18390, PDB-8qgt: 5'vRNA-bound Hantaan virus polymerase in monomeric intermediate state 手法: EM (単粒子) / 解像度: 2.8 Å 18391 8qgu EMDB-18391: Chimeric map of apo Hantaan virus polymerase in dimeric state PDB-8qgu: Apo Hantaan virus polymerase in dimeric state 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-18392: Hantaan virus polymerase Apo Dimer 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-18393: Focused map used to visualize the endonuclease of Hantaan virus polymerase apo dimer 手法: EM (単粒子) / 解像度: 3.12 Å EMDB-18394: Focused map of Hantaan virus polymerase dimer used to refine the cap-binding domain 手法: EM (単粒子) / 解像度: 3.2 Å 18397 8qh3 EMDB-18397, PDB-8qh3: 5'vRNA-bound Hantaan virus polymerase in monomeric active state 手法: EM (単粒子) / 解像度: 2.81 Å EMDB-18405: Cryo-EM map focused on the external protomers and one internal protomer of Hantaan virus polymerase hexamer 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18406: Cryo-EM of Hantaan virus polymerase in hexameric state 手法: EM (単粒子) / 解像度: 3.24 Å 18408 8qhd EMDB-18408: Chimeric cryo-EM map of Hantaan virus polymerase in hexameric state PDB-8qhd: Hantaan virus polymerase in hexameric state 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	hantaan virus 76-118 (ウイルス)
キーワード	VIRAL PROTEIN / Bunyavirus / Hantaan virus / polymerase / dimer