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-Structure paper
タイトル | Structural basis of DNA crossover capture by DNA gyrase. |
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ジャーナル・号・ページ | Science, Vol. 384, Issue 6692, Page 227-232, Year 2024 |
掲載日 | 2024年4月12日 |
著者 | Marlène Vayssières / Nils Marechal / Long Yun / Brian Lopez Duran / Naveen Kumar Murugasamy / Jonathan M Fogg / Lynn Zechiedrich / Marc Nadal / Valérie Lamour / |
PubMed 要旨 | DNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex ...DNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex through the transient double-stranded break of the other, remains elusive owing to structures derived solely from single linear duplex DNAs lacking topological constraints. Using cryo-electron microscopy, we solved the structure of DNA gyrase bound to a negatively supercoiled minicircle DNA. We show how DNA gyrase captures a DNA crossover, revealing both conserved molecular grooves that accommodate the DNA helices. Together with molecular tweezer experiments, the structure shows that the DNA crossover is of positive chirality, reconciling the binding step of gyrase-mediated DNA relaxation and supercoiling in a single structure. |
リンク | Science / PubMed:38603484 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.9 - 4.2 Å |
構造データ | EMDB-18342, PDB-8qdx: EMDB-18565: E. coli DNA gyrase bound to a DNA crossover EMDB-18566: Focused map of GyrA-CTD and T-segment DNA from the DNA crossover-gyrase complex EMDB-18567: Focused map of GyrA-CTD from DNA crossover-gyrase complex EMDB-18603, PDB-8qqs: EMDB-18605, PDB-8qqu: |
由来 |
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キーワード | ISOMERASE / DNA-protein complex / type IIA topoisomerase DNA gyrase / type IIA topoisomerase / DNA gyrase |