EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Activation mechanism of a short argonaute-TIR prokaryotic immune system.
ジャーナル・号・ページ	Sci Adv, Vol. 9, Issue 29, Page eadh9002, Year 2023
掲載日	2023年7月21日
著者	Dongchun Ni / Xuhang Lu / Henning Stahlberg / Babatunde Ekundayo /
PubMed 要旨	Short prokaryotic argonaute (pAgo) and toll/interleukin-1 receptor/resistance protein (TIR)-analog of PAZ (APAZ) form a heterodimeric SPARTA complex that provides immunity to its prokaryotic host ...Short prokaryotic argonaute (pAgo) and toll/interleukin-1 receptor/resistance protein (TIR)-analog of PAZ (APAZ) form a heterodimeric SPARTA complex that provides immunity to its prokaryotic host through an abortive infection mechanism. Monomeric SPARTA senses foreign RNA/DNA duplexes to assemble an active tetramer resulting in cell death by nicotinamide adenine dinucleotide (oxidized form) (NAD) depletion via an unknown mechanism. We report nine structures of SPARTA in different functional states at a resolution range of 4.2 to 2.9 angstroms, revealing its activation mechanism. Inactive SPARTA monomers bind to RNA/DNA duplexes to form symmetric dimers mediated by the association of Ago subunits. The initiation of tetramer assembly induces flexibility of the TIR domains enabling a symmetry-breaking rotational movement of a TIR domain in the dimer units which facilitates the TIR oligomerization, resulting in the formation of the substrate binding pocket and the activation of the SPARTA complex's NADase activity. Our findings provide detailed structural and mechanistic insights into activating a short argonaute defense system.
リンク	Sci Adv / PubMed:37467330 / PubMed Central
手法	EM (単粒子)
解像度	2.91 - 4.85 Å
構造データ	17299 8oz6 EMDB-17299, PDB-8oz6: cryoEM structure of SPARTA complex ligand-free 手法: EM (単粒子) / 解像度: 3.97 Å EMDB-17300: Duplex-bound tetramer high-resolution (EMDB-xxxx) SPARTA complex 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-17301: Dimer-1 SPARTA complex 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-17302: Dimer-2 SPARTA complex 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-17303: monomer-duplex bound 手法: EM (単粒子) / 解像度: 4.85 Å 17304 8ozc EMDB-17304, PDB-8ozc: cryoEM structure of SPARTA complex heterodimer apo 手法: EM (単粒子) / 解像度: 4.0 Å 17305 8ozd EMDB-17305, PDB-8ozd: cryoEM structure of SPARTA complex dimer-3 手法: EM (単粒子) / 解像度: 3.89 Å 17306 8oze EMDB-17306, PDB-8oze: cryoEM structure of SPARTA complex dimer high resolution 手法: EM (単粒子) / 解像度: 2.91 Å 17307 8ozf EMDB-17307, PDB-8ozf: cryoEM structure of SPARTA complex Tetramer Post-NAD cleavage-2 手法: EM (単粒子) / 解像度: 3.73 Å 17308 8ozg EMDB-17308, PDB-8ozg: cryoEM structure of SPARTA complex Tetramer Post-NAD cleavage-1 手法: EM (単粒子) / 解像度: 3.37 Å 17310 8ozi EMDB-17310, PDB-8ozi: cryoEM structure of SPARTA complex pre-NAD cleavage 手法: EM (単粒子) / 解像度: 3.22 Å
化合物	ChemComp-AR6: [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM
由来	maribacter polysiphoniae (バクテリア)
キーワード	ANTIVIRAL PROTEIN / SPARTA / TIR / prokaryotic argonaute / DNA BINDING PROTEIN / IMMUNE SYSTEM