+検索条件
-Structure paper
タイトル | 4.6A Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300 keV on a 4k x 4k CCD camera. |
---|---|
ジャーナル・号・ページ | J Struct Biol, Vol. 171, Issue 3, Page 303-308, Year 2010 |
掲載日 | 2010年6月15日 |
著者 | Daniel K Clare / Elena V Orlova / |
PubMed 要旨 | Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a ...Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV. |
リンク | J Struct Biol / PubMed:20558300 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 4.6 Å |
構造データ | |
由来 |
|
キーワード | VIRUS / SINGLE PARTICLE PROCESSING |