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-Structure paper
タイトル | Coupled chaperone action in folding and assembly of hexadecameric Rubisco. |
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ジャーナル・号・ページ | Nature, Vol. 463, Issue 7278, Page 197-202, Year 2010 |
掲載日 | 2010年1月14日 |
著者 | Cuimin Liu / Anna L Young / Amanda Starling-Windhof / Andreas Bracher / Sandra Saschenbrecker / Bharathi Vasudeva Rao / Karnam Vasudeva Rao / Otto Berninghausen / Thorsten Mielke / F Ulrich Hartl / Roland Beckmann / Manajit Hayer-Hartl / |
PubMed 要旨 | Form I Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase), a complex of eight large (RbcL) and eight small (RbcS) subunits, catalyses the fixation of atmospheric CO(2) in photosynthesis. The ...Form I Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase), a complex of eight large (RbcL) and eight small (RbcS) subunits, catalyses the fixation of atmospheric CO(2) in photosynthesis. The limited catalytic efficiency of Rubisco has sparked extensive efforts to re-engineer the enzyme with the goal of enhancing agricultural productivity. To facilitate such efforts we analysed the formation of cyanobacterial form I Rubisco by in vitro reconstitution and cryo-electron microscopy. We show that RbcL subunit folding by the GroEL/GroES chaperonin is tightly coupled with assembly mediated by the chaperone RbcX(2). RbcL monomers remain partially unstable and retain high affinity for GroEL until captured by RbcX(2). As revealed by the structure of a RbcL(8)-(RbcX(2))(8) assembly intermediate, RbcX(2) acts as a molecular staple in stabilizing the RbcL subunits as dimers and facilitates RbcL(8) core assembly. Finally, addition of RbcS results in RbcX(2) release and holoenzyme formation. Specific assembly chaperones may be required more generally in the formation of complex oligomeric structures when folding is closely coupled to assembly. |
リンク | Nature / PubMed:20075914 |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.3 - 17.0 Å |
構造データ | EMDB-1654: Rubisco RbcL8-RbcX2-8 complex EMDB-1655: Coupled chaperone action in folding and assembly of hexadecameric Rubisco EMDB-1656: Control structure of the RbcL8 octamer PDB-3hyb: |
化合物 | ChemComp-SO4: ChemComp-HOH: |
由来 |
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キーワード | PHOTOSYNTHESIS / COMPLEX ASSEMBLY / PHOTORESPIRATION / DISULFIDE BOND / CARBON FIXATION / LYASE / CHAPERONE / CALVIN CYCLE / CARBON DIOXIDE FIXATION / MONOOXYGENASE / METAL-BINDING / OXIDOREDUCTASE / RuBisCO / protein complex assembly |