EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM captures early ribosome assembly in action.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 898, Year 2023
掲載日	2023年2月17日
著者	Bo Qin / Simon M Lauer / Annika Balke / Carlos H Vieira-Vieira / Jörg Bürger / Thorsten Mielke / Matthias Selbach / Patrick Scheerer / Christian M T Spahn / Rainer Nikolay /
PubMed 要旨	Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal ...Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal proteins. To obtain insights into the still unexplored early assembly phase of the bacterial 50S subunit, we exploited a minimal in vitro reconstitution system using purified ribosomal components and scalable reaction conditions. Time-limited assembly assays combined with cryo-EM analysis visualizes the structurally complex assembly pathway starting with a particle consisting of ordered density for only ~500 nucleotides of 23S rRNA domain I and three ribosomal proteins. In addition, our structural analysis reveals that early 50S assembly occurs in a domain-wise fashion, while late 50S assembly proceeds incrementally. Furthermore, we find that both ribosomal proteins and folded rRNA helices, occupying surface exposed regions on pre-50S particles, induce, or stabilize rRNA folds within adjacent regions, thereby creating cooperativity.
リンク	Nat Commun / PubMed:36797249 / PubMed Central
手法	EM (単粒子)
解像度	3.03 - 6.62 Å
構造データ	16494 8c8x EMDB-16494, PDB-8c8x: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.93 Å 16495 8c8y EMDB-16495, PDB-8c8y: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.03 Å 16496 8c8z EMDB-16496, PDB-8c8z: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.12 Å 16497 8c90 EMDB-16497, PDB-8c90: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.15 Å 16498 8c91 EMDB-16498, PDB-8c91: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 4.19 Å 16499 8c92 EMDB-16499, PDB-8c92: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.79 Å 16500 8c93 EMDB-16500, PDB-8c93: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 4.17 Å 16501 8c94 EMDB-16501, PDB-8c94: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.8 Å 16502 8c95 EMDB-16502, PDB-8c95: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 4.92 Å 16503 8c96 EMDB-16503, PDB-8c96: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 4.43 Å 16504 8c97 EMDB-16504, PDB-8c97: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 4.07 Å 16505 8c98 EMDB-16505, PDB-8c98: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.66 Å 16506 8c99 EMDB-16506, PDB-8c99: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 3.29 Å 16507 8c9a EMDB-16507, PDB-8c9a: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 4.86 Å 16508 8c9b EMDB-16508, PDB-8c9b: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 5.9 Å 16509 8c9c EMDB-16509, PDB-8c9c: Cryo-EM captures early ribosome assembly in action 手法: EM (単粒子) / 解像度: 6.62 Å
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME / ribosome assembly / ribosome biogenesis / total reconstitution / RNA / ribosomal protein.