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-Structure paper
タイトル | Impact of the yeast S0/uS2-cluster ribosomal protein rpS21/eS21 on rRNA folding and the architecture of small ribosomal subunit precursors. |
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ジャーナル・号・ページ | PLoS One, Vol. 18, Issue 3, Page e0283698, Year 2023 |
掲載日 | 2023年3月30日 |
著者 | Gisela Pöll / Joachim Griesenbeck / Herbert Tschochner / Philipp Milkereit / |
PubMed 要旨 | RpS0/uS2, rpS2/uS5, and rpS21/eS21 form a cluster of ribosomal proteins (S0-cluster) at the head-body junction near the central pseudoknot of eukaryotic small ribosomal subunits (SSU). Previous work ...RpS0/uS2, rpS2/uS5, and rpS21/eS21 form a cluster of ribosomal proteins (S0-cluster) at the head-body junction near the central pseudoknot of eukaryotic small ribosomal subunits (SSU). Previous work in yeast indicated that S0-cluster assembly is required for the stabilisation and maturation of SSU precursors at specific post-nucleolar stages. Here, we analysed the role of S0-cluster formation for rRNA folding. Structures of SSU precursors isolated from yeast S0-cluster expression mutants or control strains were analysed by cryogenic electron microscopy. The obtained resolution was sufficient to detect individual 2'-O-methyl RNA modifications using an unbiased scoring approach. The data show how S0-cluster formation enables the initial recruitment of the pre-rRNA processing factor Nob1 in yeast. Furthermore, they reveal hierarchical effects on the pre-rRNA folding pathway, including the final maturation of the central pseudoknot. Based on these structural insights we discuss how formation of the S0-cluster determines at this early cytoplasmic assembly checkpoint if SSU precursors further mature or are degraded. |
リンク | PLoS One / PubMed:36996028 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.7 - 4.3 Å |
構造データ | EMDB-16346: Enp1TAP-S21_A, consensus Refinement EMDB-16347, PDB-8c00: EMDB-16348: Enp1TAP_A, consensus refinement EMDB-16349: Enp1TAP_A population of yeast small ribosomal subunit precursors, multibody refinement EMDB-16350: Enp1TAP_B, multibody refinement EMDB-16351: Enp1TAP_B, consensus refinement EMDB-16352: Slx9TAP-S21_A EMDB-16358: Enp1TAP_B, body 1 EMDB-16359: Enp1TAP_B, body 2 EMDB-16360: Enp1TAP_A, body 1 EMDB-16361: Enp1TAP_A, body 2 EMDB-16362: Enp1TAP-S21_A, body 1 EMDB-16363: Enp1TAP-S21_A, body 2 |
化合物 | ChemComp-ZN: |
由来 |
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キーワード | RIBOSOME / ribosomal assembly state |