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-Structure paper
タイトル | Structure of the type III-D CRISPR effector. |
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ジャーナル・号・ページ | Curr Res Struct Biol, Vol. 5, Page 100098, Year 2023 |
掲載日 | 2023年2月10日 |
![]() | Giuseppe Cannone / Dmytro Kompaniiets / Shirley Graham / Malcolm F White / Laura Spagnolo / ![]() |
PubMed 要旨 | CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 ...CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a range of enzymatic activities and downstream ancillary effectors. The broad array of current biotechnological CRISPR applications is mainly based on proteins classified as Type II, however recent developments established the feasibility and efficacy of multi-protein Type III CRISPR-Cas effector complexes as RNA-targeting tools in eukaryotes. The crenarchaeon has two type III system subtypes (III-B and III-D). Here, we report the cryo-EM structure of the Csm Type III-D complex from (SsoCsm), which uses CRISPR RNA to bind target RNA molecules, activating the Cas10 subunit for antiviral defence. The structure reveals the complex organisation, subunit/subunit connectivity and protein/guide RNA interactions of the SsoCsm complex, one of the largest CRISPR effectors known. |
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手法 | EM (単粒子) |
解像度 | 3.5 - 4.0 Å |
構造データ | EMDB-16126, PDB-8bmw: ![]() EMDB-16174: Saccharolobus solfataricus type III-D CRISPR effector - Body 1 ![]() EMDB-16175: Saccharolobus solfataricus type III-D CRISPR effector body 2 ![]() EMDB-16176: Saccharolobus solfataricus type III-D CRISPR effector body 3 ![]() EMDB-16177: Saccharolobus solfataricus type III-D CRISPR effector Body 4 ![]() EMDB-16679: SsoCsm consensus |
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![]() | RNA BINDING PROTEIN / Sulfolobus / Type III CRISPR |