EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Expulsion mechanism of the substrate-translocating subunit in ECF transporters.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4484, Year 2023
掲載日	2023年7月25日
著者	Chancievan Thangaratnarajah / Mark Nijland / Luís Borges-Araújo / Aike Jeucken / Jan Rheinberger / Siewert J Marrink / Paulo C T Souza / Cristina Paulino / Dirk J Slotboom /
PubMed 要旨	Energy-coupling factor (ECF)-type transporters mediate the uptake of micronutrients in many bacteria. They consist of a substrate-translocating subunit (S-component) and an ATP-hydrolysing motor (ECF ...Energy-coupling factor (ECF)-type transporters mediate the uptake of micronutrients in many bacteria. They consist of a substrate-translocating subunit (S-component) and an ATP-hydrolysing motor (ECF module) Previous data indicate that the S-component topples within the membrane to alternately expose the binding site to either side of the membrane. In many ECF transporters, the substrate-free S-component can be expelled from the ECF module. Here we study this enigmatic expulsion step by cryogenic electron microscopy and reveal that ATP induces a concave-to-convex shape change of two long helices in the motor, thereby destroying the S-component's docking site and allowing for its dissociation. We show that adaptation of the membrane morphology to the conformational state of the motor may favour expulsion of the substrate-free S-component when ATP is bound and docking of the substrate-loaded S-component after hydrolysis. Our work provides a picture of bilayer-assisted chemo-mechanical coupling in the transport cycle of ECF transporters.
リンク	Nat Commun / PubMed:37491368 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 4.3 Å
構造データ	16120 8bmp EMDB-16120, PDB-8bmp: Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to ATP and ADP 手法: EM (単粒子) / 解像度: 3.2 Å 16121 8bmq EMDB-16121, PDB-8bmq: Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to AMP-PNP 手法: EM (単粒子) / 解像度: 3.6 Å 16122 8bmr EMDB-16122, PDB-8bmr: Cryo-EM structure of the wild-type solitary ECF module in MSP2N2 lipid nanodiscs in the ATPase open and nucleotide-free conformation 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-16123: Cryo-EM structure of the wild-type solitary ECF module in DDM micelles in the ATPase open and nucleotide-free conformation 手法: EM (単粒子) / 解像度: 4.3 Å 16124 8bms EMDB-16124, PDB-8bms: Cryo-EM structure of the mutant solitary ECF module 2EQ in MSP2N2 lipid nanodiscs in the ATPase closed and ATP-bound conformation 手法: EM (単粒子) / 解像度: 2.6 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-HOH*: WATER
由来	lactobacillus delbrueckii subsp. bulgaricus atcc 11842 = jcm 1002 (乳酸菌)
キーワード	MEMBRANE PROTEIN / ABC Transporter / ECF transporter complex / ATP / ADP / AMP-PNP / motor