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-Structure paper
タイトル | Structures of the eukaryotic ribosome and its translational states in situ. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 7435, Year 2022 |
掲載日 | 2022年12月2日 |
著者 | Patrick C Hoffmann / Jan Philipp Kreysing / Iskander Khusainov / Maarten W Tuijtel / Sonja Welsch / Martin Beck / |
PubMed 要旨 | Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. ...Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. Different translational states of ribosomes have been well characterized in vitro. However, to which extent the known translational states are representative of the native situation inside cells has thus far only been addressed in prokaryotes. Here, we apply cryo-electron tomography to cryo-FIB milled Dictyostelium discoideum cells combined with subtomogram averaging and classification. We obtain an in situ structure that is locally resolved up to 3 Angstrom, the distribution of eukaryotic ribosome translational states, and unique arrangement of rRNA expansion segments. Our work demonstrates the use of in situ structural biology techniques for identifying distinct ribosome states within the cellular environment. |
リンク | Nat Commun / PubMed:36460643 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 3.8 - 16.0 Å |
構造データ | EMDB-15807: In situ Dictyostelium discoideum 80S ribosome from dataset2 (1.223A/px) EMDB-15808: In situ Dictyostelium discoideum 80S ribosome from dataset1 (2.176A/px) EMDB-15809: In situ Dictyostelium discoideum membrane-bound 80S ribosome EMDB-15810: In situ Dictyostelium discoideum 80S ribosome with eEF1A, A/T-tRNA and P-tRNA bound EMDB-15811: In situ Dictyostelium discoideum 80S ribosome with eEF1A, A/T-tRNA, P-tRNA and E-tRNA bound EMDB-15812: In situ Dictyostelium discoideum 80S ribosome with A-tRNA and P-tRNA bound EMDB-15813: In situ Dictyostelium discoideum 80S ribosome with P-tRNA bound EMDB-15814: In situ Dictyostelium discoideum 80S ribosome with factor, A-tRNA and P-tRNA bound EMDB-15815: In situ Dictyostelium discoideum 80S ribosome with eEF2, aa/P-tRNA and P/E-tRNA bound EMDB-15816: In situ Dictyostelium discoideum 80S ribosome with eEF2, ap/P-tRNA and P/E-tRNA bound EMDB-15843: In situ Dictyostelium discoideum 80S ribosome with eEF2 bound (substate1) EMDB-15844: In situ Dictyostelium discoideum 80S ribosome with eEF2 bound (substate2) EMDB-15845: In situ Dictyostelium discoideum 80S ribosome with eEF2, P-tRNA, and E-tRNA bound |
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